Skip to main content
SpringerLink
Log in

Successful fertilization, embryo development, and pregnancy in human in vitro fertilization (IVF) using a chemically defined culture medium containing no protein

  • Special Contributions
  • Published:
Journal of in Vitro Fertilization and Embryo Transfer Aims and scope Submit manuscript

Abstract

A randomized control trial involving the fertilization and culture of human embryos in culture medium (T6) conraining either 10% maternal serum or no protein or amino acid supplement was carried out to assess the effect of deletion from culture of all fixed nitrogen on fertilization, embryo development, and embryo viability. There was no difference in fertilization rates (68 vs 69%), development of apparently normal embryos (96 vs 97%), pregnancy rate (18 vs 14%), or birth rate (13 vs 11%) between protein-containing and protein-free media. Deletion of protein from the culture medium may enable the constitution of more appropriate and defined culture media for human in vitro fertilization (IVF).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Cholewa JA, Whitten WK: Development of 2-cell mouse embryos in vitro. III. The effect of fixed nitrogen source. J Exp Zool 1965;158:69–78

    Google Scholar 

  2. Caro CM, Trounson A: The effect of protein on preimplantation mouse embryo development in vitro. J Vitro Fert Embryo Transfer 1984;1:183–187

    Google Scholar 

  3. Shirley B, Wortham JWE, Witmyer J, Condon-Mahony M, Fort G: Effects of human serum and plasma on development of mouse embryos in culture medium. Fertil Steril 1985;43:129–134

    Google Scholar 

  4. Trounson A, Calabrese R: Changes in plasma progesterone concentrations around the time of the Luteinizing Hormone surge in women superovulated for in vitro fertilization. J Clin Endocrinol Metab 1984;59:1075–1080

    Google Scholar 

  5. Trounson AO, Mohr LR, Wood C, Leeton JF: Effect of delayed insemination on in vitro fertilization, culture and transfer of human embryos. J Reprod Fert 1982;64:285–294

    Google Scholar 

  6. Mahadevan M, Trounson AO: The influence of seminal characteristics on the success rate of human in vitro fertilization. Fertil Steril 1984;42:400–405

    Google Scholar 

  7. Leeton J, Trounson A, Jessup D, Wood C: The technique of human embryo transfer. Fertil Steril 1982;38:156–160

    Google Scholar 

  8. Menezo Y, Testart J, Perrone D: Serum is not necessary for human in vitro fertilization, early embryo culture and transfer. Fertil Steril 1984;42:750–755

    Google Scholar 

  9. Trounson A: In vitro fertilization and embryo preservation.In In Vitro Fertilization and Embryo Transfer, A Trounson, C Wood (eds). Edinburgh, Churchill Livingstone 1984, pp 111–130

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Centre for Early Human Development, Monash University, Queen Victoria Medical Centre, 172 Lonsdale Street, 3000, Melbourne, Australia

    Catriona M. Caro & Alan Trounson

Authors
  1. Catriona M. Caro
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Alan Trounson
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Caro, C.M., Trounson, A. Successful fertilization, embryo development, and pregnancy in human in vitro fertilization (IVF) using a chemically defined culture medium containing no protein. J Assist Reprod Genet 3, 215–217 (1986). https://doi.org/10.1007/BF01132806

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01132806

Key Words

Search

Navigation