Summary
Several strains of the enterobacterial groupErwinia herbicola (Enterobacter agglomerans) were screened for siderophore production. After 3 days of growth in a low-iron medium, all strains studied produced hydroxamate siderophores. The retention values of the main siderophore during thin-layer chromatography on silica gel plates and on HPLC reversed-phase columns were identical with those of an authentic sample of ferrioxamine E (norcardamine). Gas-chromatographic analysis of the HI hydrolyzate yielded succinic acid and 1,5-diaminopentane in equimolar amounts; fast-atom-bombardment (FAB) mass spectroscopy showed a molecular mass of 653 Da. Iron from55Fe-labelled ferrioxamine E was well taken up by iron-starved cells ofE. herbicola (K m=0.1 μM,V max=8 pmol mg−1 min−1). However, besides ferrioxamine E (100%), several exogenous siderophores such as enterobactin (94.5%), ferric citrate (78.5%), coprogen (63.5%) and ferrichrome (17.5%) served as siderophores, suggesting the presence of multiple siderophore receptors in the outer membrane ofE. herbicola.
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Berner, I., Konetschny-Rapp, S., Jung, G. et al. Characterization of ferrioxamine E as the principal siderophore ofErwinia herbicola (Enterobacter agglomerans) . Biol Metals 1, 51–56 (1988). https://doi.org/10.1007/BF01128017
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DOI: https://doi.org/10.1007/BF01128017