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Aldehyde fuchsin staining of pancreatic B cells. Reproducible high-contrast staining of formalin-fixed and paraffin-embedded material

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Summary

The use of Aldehyde Fuchsin for the demonstration of B cell granules in the pancreatic islets on material fixed in formalin and embedded in paraffin has led to variable results.

Treatment of such sections for 1 h with Bouin's fluid or 5% glutaraldehyde prior to deparaffinization, however, stabilizes the secretory granules in B cells. In addition, the zymogenic granules of the acinar cells exhibit increased stainability with the permanganate Aldehyde Fuchsin procedure.

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Authors and Affiliations

  1. Department of Pathology, Hvidovre Hospital, University of Copenhagen, Kettegård allé 30, 2650, Hvidovre, Denmark

    Hans Lyon

  2. Institute of General Zoology, University of Copenhagen, Universitetsparken 15, 2100, Copenhagen Ø, Denmark

    Poul Prentø

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  1. Hans Lyon
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  2. Poul Prentø
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Lyon, H., Prentø, P. Aldehyde fuchsin staining of pancreatic B cells. Reproducible high-contrast staining of formalin-fixed and paraffin-embedded material. Histochem J 12, 97–105 (1980). https://doi.org/10.1007/BF01066540

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  • DOI: https://doi.org/10.1007/BF01066540

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