Skip to main content
SpringerLink
Log in

Electrophoretic karyotype ofTrichoderma reesei

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Summary

Pulsed field electrophoresis was used to separate the intact chromosomes of the fungi imperfectiTrichoderma reesei for which no previous karyotype has been available.T. reesei was shown to have six chromosomes with molecular weights ranging from 3.3 to 11.9 megabasepairs (Mbp). The genome size was estimated to be 38 Mbp, indicating thatTrichoderma has a genome larger thanAspergillus nidulans (31 Mbp) but smaller thanNeurospora crassa (47 Mbp).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature Cited

  • Brody, H. and J. Carbon. 1989.PNAS, USA 86: 6260–6263.

    Google Scholar 

  • Gruber, F., J. Visser, C. Kubicek, and L. de Graaff. 1990.Current Genetics 18: 71–76.

    Google Scholar 

  • Harkki, A., J. Uusitalo, M. Bailey, M. Pentillä, and J. Knowles. 1989.Bio/Technology 7: 596–603.

    Google Scholar 

  • Chu, G., D. Vollrath, and R. Davis. 1986.Science 234: 1582–1585.

    Google Scholar 

  • Kubicek, C., D. Eveleigh, H. Esterbaur, W. Steiner, and C. Kubicek-Pranz (eds.). 1990.Trichoderma reesei Cellulases: Biochemistry, Genetics, Physiology and Application Royal Society of Chemistry/Cambridge.

    Google Scholar 

  • Orbach, M., D. Vollrath R. Davis, and C. Yanofsky. 1988.Molecular and Cellular Biology 8: 1469–1473.

    Google Scholar 

  • Pentillä, M., H. Nevalainen, M. Rättö, E. Salminen, and J. Knowles. 1987.Gene 61: 155–164.

    Google Scholar 

  • Schwartz, D. and C. Cantor. 1984.Cell 37: 67–75.

    Google Scholar 

  • Smith, C., T. Matsumoto, O. Niwa, S. Klco, J. Fan, M. Yanagida, and C. Cantor. 1987.Nucleic Acids Research 15: 4481–4489.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Product Development Department, Connaught Laboratories, Inc., 18370, Swiftwater, PA, USA

    John A. Gilly

  2. Department of Molecular Biology, Lehigh University, 18015, Bethlehem, PA, USA

    Jeffrey A. Sands

Authors
  1. John A. Gilly
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jeffrey A. Sands
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gilly, J.A., Sands, J.A. Electrophoretic karyotype ofTrichoderma reesei . Biotechnol Lett 13, 477–482 (1991). https://doi.org/10.1007/BF01049203

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01049203

Keywords

Search

Navigation