Abstract
A reduced tetrasaccharide chain was obtained from human von Willebrand factor (vWF) by mild alkaline borohydride treatment. The purification of thisO-glycosidically-linked oligosaccharide was achieved by serial affinity chromatography on immobilized concanavalin A andLens culinaris agglutinin and finally gel filtration. Its structure was determined by a combination of methylation studies and 500 MHz1H-NMR spectroscopy to be: NeuAc(α2-3)Gal(β1-3)[NeuAc(α2-6)]GalNAc-ol.
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Abbreviations
- ConA:
-
concanavalin A
- LCA:
-
Lens culinaris agglutinin
- vWF:
-
von Willebrand factor
- NeuAc:
-
N-acetylneuraminic acid
- Gal:
-
d-galactose
- GalNAc-ol:
-
N-acetyl-d-galactosaminitol
- HMW:
-
high molecular weight
- LMW:
-
low molecular weight
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Samor, B., Michalski, JC., Mazurier, C. et al. Primary structure of the majorO-glycosidically linked carbohydrate unit of human von Willebrand factor. Glycoconjugate J 6, 263–270 (1989). https://doi.org/10.1007/BF01047846
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DOI: https://doi.org/10.1007/BF01047846