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Effect of arachidonic acid on [3H]d-aspartate outflow in the rat hippocampus

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Abstract

The aim of this study was to investigate the effect of arachidonic acid on [3H]d-aspartate outflow in rat hippocampus synaptosomes and slices. Arachidonic acid 1) increased basal outflow of [3H]d-aspartate in both synaptosomes and slices, and 2) increased K+-evoked overflow in slices but not in synaptosomes. The latter effect was dependent (at least in part) on arachidonic acid metabolism, most likely mediated by lipo-oxygenase metabolites and free radical production. It was prevented by nordihydroguaiaretic acid but not by indomethacin, and was significantly reduced by free radical scavengers (superoxide-desmutase and catalase). This effect was dependent upon stimulation since it could not be observed after a continuous perfusion of arachidonic acid in the absence of stimulation. Furthermore, it was long-lasting since a 30 min perfusion of arachidonic acid was sufficient to exert a significant effect on a stimulation following termination of the application.

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Authors and Affiliations

  1. Institute of Pharmacology, University of Ferrara, via Fossato di Mortara 17, 44100, Ferrara, Italy

    M. Simonato, G. Bregola, C. Bianchi & L. Beani

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  1. M. Simonato
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  2. G. Bregola
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  3. C. Bianchi
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  4. L. Beani
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Simonato, M., Bregola, G., Bianchi, C. et al. Effect of arachidonic acid on [3H]d-aspartate outflow in the rat hippocampus. Neurochem Res 19, 195–200 (1994). https://doi.org/10.1007/BF00966816

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  • DOI: https://doi.org/10.1007/BF00966816

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