Abstract
ThreePlasmodium berghei exoantigens with apparent mol. wt. of 120, 31, and 13 kDa, found in infected erythrocytes by immunofluorescence, are further characterized. These antigens, synthesized in the late trophozite and schizont stages, were released into the culture medium after schizont-infected erythrocytes were placed in culture; however, they were not found in the sera of infected animals. The 120-kDa antigen proved to be somewhat heat-stable, whereas the other two did not. A monoclonal antibody (MAb) recognizing the 13-kDa antigen cross-reacted with proteins fromP. chabaudi andP. yoelii, whereas MAbs against the other two antigens reacted only with proteins from strains ofP. berghei.
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References
Anders RF (1986) Multiple cross-reactivities amongst antigens ofPlasmodium falciparum impair the development of protective immunity against malaria. Parasite Immunol 8:529–539
Ballet JJ, Druilhe P, Brasseur P, Looareesuwan S, Chantavanich P, Tharavanij S (1986) Influence of circulating malarial antigens on cell-mediated immunity in acutePlasmodium falciparum malaria. Acta Trop 43:255–262
Bianco AE, Culvenor JG, Coppel RL, Crewther PE, McIntyre RF, Favalaro JM, Brown GV, Kemp DJ, Anders RF (1987) Putative glycophorin-binding protein is secreted from schizonts ofPlasmodium falciparum. Mol Biochem Parasitol 23:91–102
Coppel RL, Cowman AF, Lingelbach KR, Brown GV, Saint RB, Kemp DJ, Anders RF (1983) Isolate-specific S-antigen ofPlasmodium falciparum contains a repeated sequence of eleven amino acids. Nature 306:751–756
Cowman AF, Saint RB, Coppel RL, Brown GV, Anders RF, Kemp DJ (1985) Conserved sequences flank variable tandem repeats in two S-antigen genes ofPlasmodium falciparum. Cell 40:775–783
Gabriel JA, Holmquist G, Perlmann H, Berzins K, Wigzell H, Perlmann P (1986) Identification of aPlasmodium chabaudi antigen present in the membrane of ring stage infected erythrocytes. Mol Biochem Parasitol 20:67–75
Hall R, McBride J, Morgan G, Tait A, Zolg JW, Walliker D, Scaife J (1983) Antigens of the erythrocytic stages of the human malaria parasitePlasmodium falciparum detected by monoclonal antibodies. Mol Biochem Parasitol 7:247–265
Holder AA, Lockyer MJ, Odink KG, Sandhu JS, Riveros-Moreno V, Nicholls SC, Hillman Y, Davey LS, Tizard MLV, Schwarz RT, Freeman RR (1985) Primary structure of the precursor to the three major surface antigens ofPlasmodium falciparum merozoites. Nature 317:270–273
Hope IA, Mackay M, Hyde JE, Goman M, Scaife J (1985) The gene for an exported antigen of the malaria parasitePlasmodium falciparum cloned and expressed inEscherichia coli. Nucleic Acids Res 13:369–379
Howard RJ, Uni S, Aikawa M, aley SB, Leech JH, Lew AM, Wellems TE, Rener J, Taylor DW (1986) Secretion of malarial histidine-rich protein (PfHRP-II) fromPlasmodium falciparum-infected erythrocytes. J Cell Biol 103:1269–1277
Kemp DJ, Coppel RL, Anders RF (1987) Repetitive proteins and genes of malaria. Ann Rev Microbiol 41:181–208
Miller LH, Howard RJ, Carter R, Good MF, Nussenzweig V, Nussenzweig RS (1986) Research toward malaria vaccines. Science 234:1349–1356
Perkins ME (1984) Surface proteins ofPlasmodium falciparum binding to the erythrocyte receptor, glycophorin. J Exp Med 160:788–798
Perkins ME (1988) Stage-dependent processing and localization of aPlasmodium falciparum protein of 130000 molecular weight. Exp Parasitol 65:61–68
Taylor DW, Evans CB, Hennessy GW, Aley SB (1986) Use of a two-sited monoclonal antibody assay to detect a heatstable malarial antigen in the sera of mice infected withPlasmodium yoelii. Infect Immun 51:884–890
Weidanz WP (1982) Malaria and alterations in immune reactivity. Br med Bull 38:167–172
Wilson RJM, McGregor IA, Hall P, Williams K, Bartholomew R (1969) Antigens associated withPlasmodium falciparum infections in man. Lancet ii:201–205
Wiser MF (1986) Characterization of monoclonal antibodies directed against erythrocytic stage antigens ofPlasmodium berghei. Eur J Cell Biol 42:45–51
Wiser MF, Schweiger HG (1985) Cytosolic protein kinase activity associated with the maturation of the malaria parasitePlasmodium berghei. Mol Biochem Parasitol 17:179–189
Wiser MF, Schweiger HG (1986) Increased sensitivity in antigen detection during immunoblot analysis resulting from antigen enrichment via immunoprecipitation. Anal Biochem 155:71–77
Wiser MF, Leible MB, Plitt B (1988) Characterization of acidic phosphoproteins associated with the host erythrocyte membrane of erythrocytes infected withPlasmodium berghei andP. chabaudi. Mol Biochem Parasitol 27:11–22
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Wiser, M.F. Plasmodium antigens external to the parasite but within the infected erythrocyte. Parasitol Res 75, 206–211 (1989). https://doi.org/10.1007/BF00931277
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DOI: https://doi.org/10.1007/BF00931277