Abstract
The patch-clamp method was applied to the lateral membrane of late proximal tubules of the rabbit kidney. Tubule segments were cannulated on one side by a perfusion system. At the noncannulated end of the tubules, the lateral membrane was accessible to a patch pipette. In cell-attached, as well as cell-excised (presumably inside-out oriented) membrane patches, a voltage sensitive channel was observed. The open-state probability of this channel increased with depolarizing potentials. In cell-excised patches bathed with NaCl-Ringer on both sides, the single channel conductance g was 28.0±1.2 pS (n=10). With KCl-Ringer in the pipette and NaCl-Ringer in the bath g was 24.7±1.3 pS (n=7) and the current-voltage curve crossed the axis at 0 mV. Therefore, the channel does not discriminate between K+ and Na+ ions. Replacing half of NaCl by mannitol on the bath side yielded a permeability for cations about twice as high as for Cl−. The channel could be reversibly blocked by diphenylamine-2-carboxylate (DPC), whereas its inhibition by SITS was only partially reversible. In cell-attached patches, the channel was nearly inactivated at zero clamp potential, but became active when the membrane patch was depolarized. The significance of this nonselective channel for proximal tubule cell function is still unclear. It could be involved in the contraluminal exit mechanism of various anions. However, it could also play a role in cell volume regulation processes.
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Barac-Nieto M, Murer H, Kinne R (1980) Lactate-sodium cotransport in rat renal brush border membranes. Am J Physiol 239:F496-F506
Baumann K, de Rouffignac C, Roinel N, Rumrich G, Ullrich KJ (1975) Renal phosphate transport: inhomogeneity of local proximal transport rates and sodium dependence. Pflügers Arch 356:287–297
Bello-Reuss E (1982) Electrical properties of the basolateral membrane of the straight portion of the rabbit proximal renal tubule. J Physiol 326:49–63
Blatz AL, Magleby KL (1985) Single chloride-selective channels active at resting membrane potentials in cultured rat skeletal muscle. Biophys J 47:119–123
Chesnoy-Marchais D, Evans MG (1984) Two types of chloride channels in outside-out patches from Aplysia neurones. J Physiol 357:64P
Colquhoun D, Sigworth FJ (1983) Fitting and statistical analysis of single-channel records. In: Sakmann B, Neher E (eds) Single-channel recording. Plenum Press, New York London, pp 191–264
DiStefano A, Wittner M, Schlatter E, Lang HJ, Englert H, Greger R (1985) Diphenylamine-2-carboxylate, a blooker of the Cl−-conductive pathway in Cl−-transporting epithelia. Pflügers Arch 405:S95-S100
Fenwick E, Marty A, Neher E (1982) A patch-clamp study of bovine chromaffin cells and of their sensitivity to acetylcholine J Physiol 331:557–597
Fischer RS, Spring KR (1984) Intracellular activities during volume regulation by Necturus gallbladder. J Membr Biol 78:187–199
Gögelein H, Greger R (1984) Single channel recordings from basolateral and apical membranes of renal proximal tubules. Pflügers Arch 401:424–426
Gögelein H, Greger R (1986) Na+ selective channels in the apical membrane of rabbit later proximal tubules (pars recta). Pflügers Arch 406:198–203
Grantham JJ, Lowe CM, Dellasega M, Cole B (1977) Effect of hypotonic medium on K and Na content of proximal renal tubules. Am J Physiol 232:F42-F49
Greger R, Gögelein H (1986) Role of K+ conductive pathways in the nephron. Kidney Int (in press)
Hamill OP, Marty A, Neher E, Sakmann B, Sigworth FJ (1981) Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflügers Arch 391:85–100
Hanrahan JW, Alles WP, Lewis SA (1984) Basolateral anion and K channels from rabbit urinary bladder epithelium. J Gen Physiol 84:30a
Helman SI, Koeppen BM, Beyenbach KW, Baxendale LM (1985) Patch clamp studies of apical membranes of renal cortical collecting ducts. Pflügers Arch 405:71–76
Hoffmann N, Thees M, Kinne R (1976) Phosphate transport by isolated renal brush border vesicles. Pflügers Arch 362:147–156
Kolb HA, Brown CDA, Murer H (1985) Identification of a voltage-dependent anion channel in the apical membrane of a Cl−-secretory epithelium (MDCK). Pflügers Arch 403:262–265
Mauyama Y, Petersen OH (1982) Single-channel currents in isolated patches of plasma membrane from basal surface of pancreatic acini. Nature 299:159–161
Mueller P, Rudin DO, Tien HT, Wescott WC (1962) Reconstitution of cell membrane structure in vitro and its transformation into an excitable system. Nature 194:979–980
Neher E (1982) Unit conductance studies in biological membranes. Techniques in Cellular Physiol P 121:1–6
Nelson DJ, Tang JM, Palmer LG (1984) Single-channel recordings of apical membrane chloride conductance in A6 epithelial cells. J Membr Biol 80:81–89
Palmer LG, Frindt G (1985) Single channels in the apical membrane of rabbit cortical collecting tubule. Renal Physiol 9:50
Rae JL (1984) Single channel recordings from ocular epithelia obtained using a patch voltage clamp. Biophys J 45:385a
Ullrich KJ, Rumrich G, Klöss S (1982) Reabsorption of monocarboxylic acids in the proximal tubule of the rat kidney. I. Transport kinetics ofd-Lactate, Na+-dependence, pH-dependence and effect of inhibitors. Pflügers Arch 395:212–219
Ullrich KJ, Papassilliou F, Rumrich G, Fritzsch G (1985) Contraluminal phosphate transport in the proximal tubule of the rat kidney. Pflügers Arch 405:S106-S109
Ullrich KJ, Papavassiliou F (1986) Contraluminal transport of aliphatic small carboxylates in the proximal tubule of the rat kidney in situ. Pflügers Arch (submitted)
Sakmann B, Neher E (1983) Geometric parameters of pipettes and membrane patches. In: Sakmann B, Neher E (eds) Single-channel recording. Plenum Press, New York London, p 37
Van Driessche W, Zeiske W (1985) Ionic channels in epithelial cell membranes. Physiol Rev 65:833–903
Völkl H, Greger R (1985) Effects of phlorrhizin and temperature changes on basolateral membrane potential on isolated in vitro perfused proximal tubules of mouse kidney. Pflügers Arch 403:R39
Wangemann P, Wittner M, DiStefano A, Englert HC, Lang HJ, Schlatter E, Greger E (1985) Cl− channel blockers in the thick ascending limb of the loop of Henle. Structure activity relationship. Pflügers Arch 407 (Suppl 2):S128-S141
White MM, Miller C (1979) A voltage-gated anion channel from the electric organ of torpedo californica. J Biol Chem 254:10161–10166
Yellen G (1982) Single Ca2+-activated nonselective cation channels in neuroblastoma. Nature 296:357–359
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Gögelein, H., Greger, R. A voltage-dependent ionic channel in the basolateral membrane of late proximal tubules of the rabbit kidney. Pflugers Arch. 407 (Suppl 2), S142–S148 (1986). https://doi.org/10.1007/BF00584943
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DOI: https://doi.org/10.1007/BF00584943