Abstract
The binding of [3H]colchicine to soluble component prepared from carrot (Daucus carota L.) cells in suspension culture was assayed by the diethylaminoethyl(DEAE)-cellulose powder method. The binding activity was very labile and the time course of the binding indicated that the colchicine-bound complex was also unstable. The reaction was enhanced by vinblastine but lumicolchicine had no effect. The optimum temperature for the reaction was 30° C, and the colchicine binding constant was calculated to be 3.5·104 l mol−1 at 30° C.
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Okamura, S. Binding of colchicine to a soluble fraction of carrot cells grown in suspension culture. Planta 149, 350–354 (1980). https://doi.org/10.1007/BF00571169
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DOI: https://doi.org/10.1007/BF00571169