Skip to main content
Springer Nature Link
Log in

High activity of an unstable form of glucose phosphate isomerase in the mouse

  • Published:
Biochemical Genetics Aims and scope Submit manuscript

Abstract

Quantitative electrophoretic studies of the three allozymes of glucose phosphate isomerase (GPI-1) produced byGpi-1s a/Gpi-1sc heterozygous mice revealed two opposing influences on GPI-1 activity. First, the GPI-1 AC heterodimer is less stable than GPI-1 AA but more stable than the GPI-1 CC homodimer. Second, a genetic determinant that maps close to or within theGpi-1s structural gene causes elevated activity of GPI-1 AC and probably also GPI-1 CC dimers. The relative lability of these allozymes masks this elevated activity in some tissues but the effect is probably ubiquitous. The significance of these observations is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Beutler, E. (1983). Selectivity of proteases as a basis for tissue distribution of enzymes in hereditary deficiencies.Proc. Natl. Acad. Sci. USA 803767.

    Google Scholar 

  • Carter, N. D., and Parr, C. W. (1967). Isoenzymes of phosphoglucose isomerase in mice.Nature 216511.

    Google Scholar 

  • DeLorenzo, R. J., and Ruddle, F. H. (1969). Genetic control of two electrophoretic variants of glucosephosphate isomerase in the mouse (Mus musculus).Biochem. Genet. 3151.

    Google Scholar 

  • Dizik, M., and Elliot, R. W. (1977). A gene apparently determining the extent of sialylation of lysosomal α-mannosidase in mouse liver.Biochem. Genet. 1531.

    Google Scholar 

  • Levak-Svajger, B., Svajger, A., and Skreb, N. (1969). Separation of germ layers in presomite rat embryos.Experientia 251311.

    Google Scholar 

  • Lusis, A. J., Chapman, V. M., Wagenstein, R. W., and Paigen, K. (1983). Trans-acting temporal locus within the β-glucuronidase gene complex.Proc. Natl. Acad. Sci. USA 804398.

    Google Scholar 

  • McLaren, A., and Buehr, M. (1981). GPI expression in female germ cells of the mouse.Genet. Res. Cambr. 37303.

    Google Scholar 

  • Padua, R. A., Bulfield, G., and Peters, J. (1978). Biochemical genetics of a new glucosephosphate isomerase allele (Gpi-1c) from wild mice.Biochem. Genet. 16127.

    Google Scholar 

  • Paigen, K. (1979). Acid hydrolases as models of genetic control.Annu. Rev. Genet. 13417.

    Google Scholar 

  • Paigen, K. (1980). Temporal genes and other developmental regulators in mammals. In Loomis, W., and Leighton, T. (eds.),The Molecular Genetics of Development Academic Press, New York, pp. 419–470.

    Google Scholar 

  • Peterson, A., Choy, F., Wong, G., Claphoff, S., and Friar, P. (1985). Glucosephosphate isomerase (GPI-1) expression in mouse ova:Cis regulation of monomer realization.Biochem. Genet. 23827.

    Google Scholar 

  • Peterson, A. C., and Wong, G. G. (1978). Genetic regulation of glucose phosphate isomerase in mouse oocytes.Nature 276267.

    Google Scholar 

  • Russell, E. S., and Bernstein, S. E. (1966). Blood and blood formation. In Green, E. L. (ed.),Biology of the Laboratory Mouse 2nd ed., Dover, New York, pp. 351–372.

    Google Scholar 

  • Warner, C. M., Meyer, T. E., Balinsky, D., and Briggs, C. J. (1985). Variations in the amount of glucose phosphate isomerase in lymphocytes and erythrocytes from A/J and C57BL/6 mice.Biochem. Genet. 23815.

    Google Scholar 

  • West, J. D., and Fisher, G. (1984). A new allele of theGpi-1t temporal gene that regulates the expression of glucose phosphate isomerase in mouse oocytes.Genet. Res. 44169.

    Google Scholar 

  • West, J. D., and Green, J. F. (1983). The transition from oocyte-coded to embryo-coded glucose phosphate isomerase in the early mouse embryo.J. Embryol. Exp. Morphol. 78127.

    Google Scholar 

  • West, J. D., Frels, W. J., Chapman, V. M., and Papaioannou, V. E. (1977). Preferential expression of the maternally derived X-chromosome in the mouse yolk sac.Cell 12873.

    Google Scholar 

  • West, J. D., Leask, R., and Green, J. F. (1986). Quantification of the transition from oocyte-coded to embryo-coded glucose phosphate isomerase in mouse embryos.J. Embryol. Exp. Morphol. 97225.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Obstetrics and Gynaecology, University of Edinburgh, EH3 9EW, Edinburgh, Scotland

    John D. West, Rosemary Leask & Jean H. Flockhart

  2. MRC Radiobiology Unit, OX11 ORD, Chilton, Didcot, Oxon, England

    John D. West & Graham Fisher

Authors
  1. John D. West
    View author publications

    You can also search for this author inPubMed Google Scholar

  2. Rosemary Leask
    View author publications

    You can also search for this author inPubMed Google Scholar

  3. Jean H. Flockhart
    View author publications

    You can also search for this author inPubMed Google Scholar

  4. Graham Fisher
    View author publications

    You can also search for this author inPubMed Google Scholar

Additional information

This study was begun while JDW was at the MRC Radiobiology Unit and continued at the Department of Obstetrics and Gynaecology of the University of Edinburgh, where it was supported, in part, by a grant from the Moray Endowment Fund.

Rights and permissions

Reprints and permissions

About this article

Cite this article

West, J.D., Leask, R., Flockhart, J.H. et al. High activity of an unstable form of glucose phosphate isomerase in the mouse. Biochem Genet 25, 543–561 (1987). https://doi.org/10.1007/BF00554356

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00554356

Key words