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Neurotensin-like immunoreactivity in the nervous system of hydra

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Summary

Neurotensin-like immunoreactivity is found in nerve fibers present in all body regions of hydra. The nerve fibers are especially numerous in the ectoderm at the bases of the tentacles and in the ectoderm at a site just above the foot. Radioimmunoassays of acetic-acid extracts of hydra, using various region-specific antisera towards mammalian neurotensin, show the presence of multiple neurotensin-related peptides. The amounts of these peptides vary between 1 and 350 pmol per gram wet weight. Gel filtration on Sephadex G-25 reveals a fraction of neurotensin-like peptides that crossreacts equally well with an antiserum directed against sequence 1–8 and an antiserum directed against sequence 6–13 of neurotensin. This fraction elutes also at the position of neurotensin and might closely resemble the mammalian peptide. A fraction eluting with the void volume crossreacts preferentially with antisera directed against sequences 1–8 and 10–13 of neurotensin. Several components of apparent lower molecular weight than neurotensin crossreact preferentially with an antiserum against sequence 10–13. These last peptides represent the major portion of the neurotensin-like peptides in hydra.

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References

  • Araki K, Tachibana S, Uchiyama M, Nakajima T, Yasuhara T (1973) Isolation and structure of a new peptide xenopsin on the smooth muscle, especially on a strip of fundus from a rat stomach, from the skin of Xenopus laevis. Chem Pharm Bull 21:2801–2804

    Google Scholar 

  • Baumgarten HG, Holstein AF, Owman Ch (1970) Auerbach's plexus of mammals and man: Electronmicroscopic identification of three different types of neuronal processes in the myenteric ganglia of the large intestine from rhesus monkey, guinea pig and man. Z Zellforsch mikrosk Anat 106:376–397

    Google Scholar 

  • Carraway RE, Leeman SE (1973) The isolation of a new hypotensive peptide, neurotensin, from bovine hypothalami. J Biol Chem 248:6854–6861

    Google Scholar 

  • Carraway RE, Leeman SE (1975a) The amino-acid sequence of a hypothalamic peptide, neurotensin. J Biol Chem 250:1907–1911

    Google Scholar 

  • Carraway RE, Leeman SE (1975b) Structural requirements for the biological activity of neurotensin, a new vasoactive peptide. In: Walter R, Meienhof J (eds.) Peptides: Chemistry, structure and biology. Ann Arbor Science, Ann Arbor, pp 679–685

    Google Scholar 

  • Carraway RE, Leeman SE (1976a) Radioimmunoassay of neurotensin, a hypothalamic peptide. J Biol Chem 251:7035–7044

    Google Scholar 

  • Carraway RE, Leeman SE (1976b) Characterization of radioimmunoassayable neurotensin in the rat; its differnetial distribution in the central nervous system, small intestine and stomach. J Biol Chem 251:7045–7052

    Google Scholar 

  • Carraway RE, Bhatnagar M (1980) Immunochemical characterization of neurotensin-like peptides in chicken. Peptides 1:159–165

    Google Scholar 

  • Coons AH, Leduc EH, Connoly JM (1955) Studies on antibody production. I. A method for the histochemical demonstration of specific antibody and its application to the study of the hyperimmune rabbit. J Exp Med 102:49–59

    Google Scholar 

  • David CN (1973) A quantitative method for maceration of Hydra tissue. Wilhelm Roux's Archiv 171:259–268

    Google Scholar 

  • Davis LE, Burnett AL, Haynes JF (1968) Histological and ultrastructural study of the muscular and nervous systems in Hydra. J Exp Zool 167:295–332

    Google Scholar 

  • El-Salhy M, Abou-El-Ela R, Falkmer S, Grimelius L, Wilander E (1980) Immunohistochemical evidence of gastro-entero-pancreatic neurohormonal peptides of vertebrate type in the nervous system of the larva of a dipteran insect, the hoverfly Eristalis aeneus. Regulatory Peptides 1:187–204

    Google Scholar 

  • Epp L, Tardent P (1978) The distribution of nerve cells in Hydra attenuata Pall. Wilhelm Roux's Archiv. 185:185–193

    Google Scholar 

  • Falkmer S, Carraway RE, El-Salhy M, Emdin SO, Grimelius L, Rehfeld JF, Reinecke M, Schwartz TFW (1981) Phylogeny of the GEP neuroendocrine system. A review with special reference to the occurrence of CCK-like and neurotensin-like polypeptides in lower vertebrates and invertebrates. UCLA Forum Med Sci 23:21–42

    Google Scholar 

  • Fritsch HAR, Van Noorden S, Pearse AGE (1980) Substance P-, neurotensin- and bombesin-like immunoreactivities in the gill epithelium of Ciona intestinalis L. Cell Tissue Res 208:467–473

    Google Scholar 

  • Frigerio B, Ravazola M, Ito S, Buffa R, Capella C, Solcia E, Orci L (1977) Histochemical and ultrastructural identification of neurotensin cells in the dog ileum. Histochemistry 54:123–131

    Google Scholar 

  • Grimmelikhuijzen CJP, Schaller HD (1979) Hydra as a model system for the study of morphogenesis. Trends Biochem Sci 4:265–267

    Google Scholar 

  • Grimmelikhuijzen CJP, Sundler F, Rehfeld JF (1980) Gastrin/CCK-like immunoreactivity in the nervous system of coelenterates. Histochemistry 69:61–68

    Google Scholar 

  • Grimmelikhuijzen CJP, Balfe A, Emson PC, Powell D, Sundler F (1981) Substance P-like immunoreactivity in the nervous system of hydra. Histochemistry 71:325–333

    Google Scholar 

  • Hadži J (1909) Über das Nervensystem von Hydra. Arb Zool Inst Univ Wien 17:225–268

    Google Scholar 

  • Helmstaedter V, Taugner C, Feurle GE, Forssman WG (1977) Localization of neurotensin-immunoreactive cells in the small intestine of man and various mammals. Histochemistry 53:35–41

    Google Scholar 

  • Kataoka K, Taniguchi A, Shimizu H, Soda K, Okuno S, Yajima H, Kitagawa K (1978) Biological activity of neurotensin and its C-terminal partial sequences. Brain Res Bull 3: 555–557

    Google Scholar 

  • Kitabgi P, Carraway RE, Leeman SE (1976) The isolation of a tridecapeptide from bovine intestinal tissue and its partial characterization as neurotensin. J Biol Chem 251:7053–7058

    Google Scholar 

  • Kobayashi R, Brown M, Vale M (1977) Regional distribution of neurotensin and somatostatin in rat brain. Brain Res 126:584–588

    Google Scholar 

  • Langer M, Noorden S van, Polak JM, Pearse AGE (1979) Peptide hormone-like immunoreactivity in the gastrointestinal tract and endocrine pancreas of eleven teleost species. Cell Tissue Res 199:493–508

    Google Scholar 

  • Lentz TL, Barnett RJ (1965) Fine structure of the nervous system of hydra. Amer Zoologist 5:341–356

    Google Scholar 

  • Orci L, Baetens D, Rufener C, Brown M, Vale M, Guillemin R (1976) Evidence for immunoreactive neurotensin in dog intestinal mucosa. Life Sci 19:559–562

    Google Scholar 

  • Polak JM, Sullivan SN, Bloom SR, Buchan AMJ, Facer P, Brown MR, Pearse AGE (1977) Specific location of neurotensin to the N-cell in human intestine by radioimmunoassay and immunocytochemistry. Nature 270:183–184

    Google Scholar 

  • Reinecke M, Almasan K, Carraway RE, Helmstaedter V, Forssman WG (1980a) Distribution patterns of neurotensin-like immunoreactive cells in the gastrointestinal tract of higher vertebrates. Cell Tissue Res 205:383–395

    Google Scholar 

  • Reinecke M, Carraway RE, Falkmer S, Feurle GE, Forssmann WG (1980b) Occurance of neurotensin immunoreactive cells in the digestive tract of lower vertebrates and deuterostomian invertebrates. A correlated immunohistochemical and radioimmunochemical study. Cell Tissue Res 212:173–183

    Google Scholar 

  • Rosell S, Rökaeus Å (1979) The effect of ingestion of amino acids, glucose and fat on circulating neurotensin-like immunoreactivity (NTLI) in man. Acta Physiol Scand 107:263–267

    Google Scholar 

  • Schultzberg M, Hökfeld T, Nilsson G, Terenius L, Rehfeld JF, Brown M, Elde R, Goldstein M, Said S (1980) Distribution of peptide and catecholamine-containing neurons in the gastrointestinal tract of rat and guinea pig: Immunohistochemical studies with antisera to substance P, vasoactive intestinal polypeptide, enkephalins, somatostatin, gastrin/cholecystokinin, neurotensin and dopamine β-hydroxylase. Neuroscience 5:689–744

    Google Scholar 

  • Scrutton CT (1979) Early fossil cnidarians. In: House MR (ed) The origin of major invertebrate groups. Academic Press, New York, pp 161–209

    Google Scholar 

  • Sundler F, Alumets J, Håkanson R, Carraway RE (1977a) Ultrastructure of the gut-neurotensin cell. Histochemistry 53:25–34

    Google Scholar 

  • Sundler F, Håkanson R, Hammer RA, Alumets J, Carraway RE, Leeman SE, Zimmerman E (1977b) Immunohistochemical localization of neurotensin in endocrine cells of the gut. Cell Tissue Res 178:313–321

    Google Scholar 

  • Uhl GR, Kuhar MJ, Snyder SH (1977) Neurotensin: immunohistochemical localization in rat central nervous system. Proc Natl Acad Sci USA 74:4059–4063

    Google Scholar 

  • Van Noorden S, Falkmer S (1980) Gut-islet endocrinology — some evolutionary aspects. Invest Cell Pathol 3:21–35

    Google Scholar 

  • Westfall JA, Yamataka S, Enos PD (1971) Ultrastructural evidence of polarized synapses in the nervous net of hydra. J Cell Biol 51:318–323

    Google Scholar 

  • Westfall JA (1973a) Ultrastructural evidence for neuromuscular systems in coelenterates. Am Zool 13:237–246

    Google Scholar 

  • Westfall JA (1973b) Ultrastructural evidence for a granule-containing sensory-motor-interneuron in Hydra littoralis. J Ultrastruct Res 42:268–282

    Google Scholar 

  • Westfall JA, Kinnamon JC (1978) A second sensory-motor-interneuron with neurosecretory granules in Hydra. J Neurocyt 7:365–379

    Google Scholar 

Download references

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Author notes

  1. C. J. P. Grimmelikhuijzen

    Present address: Abteilung Biophysik, Max-Planck-Institut für medizinische Forschung, Jahnstraße 29, D-6900, Heidelberg, FRG

Authors and Affiliations

  1. Department of Histology, University of Lund, Lund, Sweden

    C. J. P. Grimmelikhuijzen & F. Sundler

  2. Max-Planck-Institut für medizinische Forschung, Heidelberg, Federal Republic of Germany

    C. J. P. Grimmelikhuijzen

  3. Department of Physiology, Medical Center, University of Massachusetts, Worcester, USA

    R. E. Carraway

  4. Department of Pharmacology, Karolinska Institutet, Stockholm, Sweden

    Å. Rökaeus

  5. Zoologisches Institut der Universität, Heidelberg, Federal Republic of Germany

    C. J. P. Grimmelikhuijzen

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  1. C. J. P. Grimmelikhuijzen
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  2. R. E. Carraway
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  3. Å. Rökaeus
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  4. F. Sundler
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Grimmelikhuijzen, C.J.P., Carraway, R.E., Rökaeus, Å. et al. Neurotensin-like immunoreactivity in the nervous system of hydra. Histochemistry 72, 199–209 (1981). https://doi.org/10.1007/BF00517133

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  • DOI: https://doi.org/10.1007/BF00517133

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