Summary
Thiamine pyrophosphatase (TPPase) activity in the neuronal Golgi apparatus and in glia cells was investigated using different tissue preparation techniques such as fresh frozen and fixed frozen sections, the effect of ATP as an activator and the fine structural localization of the enzyme activity in glia cell processes and the glio-vascular contact point was observed under the electron microscope.
TPPase was demonstrated in the glia cell using only a fixed frozen tissue preparation, activated by ATP with the concentration as low as 0.1 mM. On the contrary, enzyme activity was detected in the Golgi apparatus of the neuron regardless of tissue preparation, fresh or fixed frozen, or by the addition of ATP to the incubation medium.
The identity of the glia cell type showing a positive reaction for TPPase is though to be part astrocyte and part microglia.
The fine structural observation of TPPase localization at the glia cell process ending, particularly at the glio-vascular and glio-fibral (medullated fiber) contact point suggests a functional role of this enzyme for transport of metabolites through glia cell processes to neurons and/or the blood capilary, and also may play a role in regulation of co-carboxylase.
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References
Barron, K., Tuncbay, T.O.: Histochemistry of acid phosphatase and thiamine pyrophosphatase during axon reaction. Amer. J. Path. 40, 637–652 (1962)
Barron, K., Tuncbay, T.O.: Phosphatase histochemistry of feline cervical spinal cord after brachial plexectomy. Hydrolysis of beta-glycerophosphate, thiamine pyrophosphate and nucleoside diphosphates. J. Neuropath. exp. Neurol. 23, 368–386 (1964)
Cłuszcz, A.: Cytochemical structure of the human glia visualized by the reaction with thiamine pyrophosphate. Acta neuropath. (Berl.) 6, 371–375 (1966)
Cłuszcz, A., Sejmicka, K., Drab, A., Giernat, L.: Histochemical localization of four hydrolytic enzymes in tissue cultures of gliomas. Folia Histochem. Cytochem. (Krakow) 5, 263–274 (1967)
Goldfischer, S., Essner, E., Schiller, B.: Nucleoside diphosphatase and thiamine pyrophosphatase activities in the endoplasmic reticulum and Golgi apparatus. J. Histochem. Cytochem. 19, 349–360 (1971)
Hartmann, J.F.: High sodium content of cortical astrocytes. Electron microscopic evidence. Arch. Neurol. (Chic.) 15, 633–642 (1966)
Jonek, J., Konecki, J., Grzybek, H., Olkowski, Z.: Electron microscopic localization of acid phosphatase and thiamine pyrophosphatase activities in the motor neurons of exercised mice. Histochemie 23, 116–119 (1970)
Klatzo, I., Miquiel, J.: Observations on pinocytosis in nervous tissue. J. Neuropath. exp. Neurol. 19, 475–487 (1960)
Markov, D.V., Dimova, R.N.: Ultrastructural alterations of rat brain microglial cells and pericytes after chronic lead poisoning. Acta neuropath. (Berl.) 28, 25–35 (1974)
Novikoff, A.B., Essner, E., Goldfischer, S., Heus, M.: Nucleoside phosphatase activities of cytomembranes. In: The Interpretation of Ultrastructure, pp. 149–192 (ed. R.J.C. Harris). New York-London: Academic Press 1962
Novikoff, A.B., Goldfischer, S.: Nucleoside diphosphatase activity in the Golgi apparatus and its usefulness for cytological studies. Proc. nat. Acad. Sci. (Wash.) 47, 802–810 (1961)
Novikoff, P.M., Novikoff, A.B., Quintana, N., Hau, J.-J.: Golgi apparatus, GERL, and lysosomes of neurons in rat dorsal root ganglia, studies by thick section and thin section cytochemistry. J. Cell Biol. 50, 859–886 (1971)
Pomerat, C.M., Costero, I.: Normal and neoplastic brain in time-lapse cinematography. Proc. Second Int. Cong. Neuropathol. London. Part II, pp. 629–634. Amsterdam: Excerpta Medica 1955
Schiffer, D.: Histochemical study of TPPase and NDPase in the human nervous tissue, with particular reference to glia cells. Histochemie 33, 53–60 (1973)
Sjöstrand, J.: Changes of nucleoside phosphatase activity in the hypoglossal nucleus during nerve regeneration. Acta physiol. scand. 67, 219–228 (1966)
Tohyama, M., Akai, F., Sakumoto, T., Maeda, T.: Histochemical change of of thiamine pyrophosphatase activity of the central nervous system following neurotomy with special reference to glial reaction. Ann. Histochim. 17, 203–214 (1972)
Yamazaki, M., Hayaishi, O.: Allosteric properties of nucleoside diphosphatase and its identity with thiamine pyrophosphatase. J. biol. Chem. 243, 2934–2942 (1968)
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Kumamoto, T., Nakagawa, S., Yata, Y. et al. On the histochemistry of neuronal and glial TPPase. Histochemistry 47, 101–109 (1976). https://doi.org/10.1007/BF00492558
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DOI: https://doi.org/10.1007/BF00492558