Summary
A class of avirulent mutants of the plant pathogenic bacterium Pseudomonas solanacearum, strain GMI1000, resistant to acridine orange (Acrr), harbour a deletion of over 85 kb in their genome. This deletion affects, a≤1,000 kb megaplasmid which has previously been shown to be present in most of the strains of this species. In addition at least 11 out of 13 independent Tn5 insertions, leading to loss of virulence, are located on the megaplasmid. Nine of them are present in the region which is deleted from the Acrr mutants. These results suggest that the majority of virulence genes identified so far are plasmid borne.
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Banfalvi Z, Kondorosi E, Kondorosi A (1985) Rhizobium meliloti carries two megaplasmids. Plasmid 13:129–138
Batut J, Terzaghi B, Ghérardi M, Huguet M, Terzaghi E, Garnerone AM, Boistard P, Huguet T (1985) Localization of a symbiotic fix region on Rhizobium meliloti pSym megaplasmid more than 200 kilobases from the nod-nif region. Mol Gen Genet 199:232–239
Boucher C, Message B, Debieu D, Dischek C (1981) Use of P-1 incompatibility group plasmids to introduce transposons into Pseudomonas solanacearum. Phytopathology 71:639–642
Boucher CA, Barberis PA, Trigalet AP, Démery DA (1985) Transposon mutagenesis of Pseudomonas solanacearum: isolation of Tn5-induced avirulent mutants. J Gen Microbiol 131:2449–2457
Buddenhagen I, Kelman A (1964) Biological and physiological aspects of bacterial wilt caused by Pseudomonas solanacearum. Ann Rev Phytapathol 2:203–230
Burkardt E, Burkardt HJ (1984) Visualization and exact molecular weight determination of a Rhizobium meliloti megaplasmid. J Mol Biol 175:213–218
Cami B, Kourilsky P (1978) Screening of cloned recombinant DNA in bacteria by in situ colony hybridization. Nucleic Acids Res 5:2381–2390
Comai L, Kosuge T (1980) Involvement of plasmid deoxyribonucleic acid in indolacetic acid synthesis in Pseudomonas savastanoi. J Bacteriol 143:950–957
Dagert M, Ehrlich SD (1979) Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells. Gene 6:23–28
Ditta G, Stanfield S, Corbin D, Helinski D (1980) Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc Natl Acad Sci USA 77:7347–7351
Driguès P, Démery-Lafforgue D, Trigalet A, Dupin P, Samain D, Asselineau J (1985) Comparative studies of lipopolysaccharide and exopolysaccharide from a virulent strain of Pseudomonas solanacearum and from three avirulent mutants. J Bacteriol 162:504–509
Friedman AM, Long SR, Brown SE, Buikema WJ, Ausubel FM (1982) Construction of a broad host range cosmid cloning vector and its use in the genetic analysis of Rhizobium mutants. Gene 18:289–296
Hendrick CA, Sequeira L (1984) Lipopolysaccharide-defective mutants of the wilt pathogen Pseudomonas solanacearum. Appl Environ Microbiol 48:94–101
Hirota Y (1960) The effect of acridine dyes on mating type factors in Escherichia coli. Genetics 46:57
Husain A, Kelman A (1958) The role of pectic and celluloytic enzymes in pathogenesis by Pseudomonas solanacearum. Phytopathology 48:377–386
Kelman A (1953) The bacterial wilt caused by Pseudomonas solanacearum. North Carolina Agricultural Experiment Station Technical Bulletin 99
Kelman A (1954) The relationship of pathogenicity in Pseudomonas solanacearum to colony appearance on a tetrazolium medium. Phytopathology 44:693–695
Kerr A, Manigault P, Tempé J (1977) Transfer of virulence in vivo and in vitro in Agrobacterium. Nature 265:560–561
Long SR (1984) Genetics of Rhizobium nodulation. In: Kosuge T, Nester E (eds) Plant-microbe interaction. Macmillan Publishing Co, NY, pp 265–306
Maniatis T, Fritsch E, Sambrook J (1982) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Message B, Boistard P, Pitrat M, Schmit J, Boucher C (1978) A new class of fluidal avirulent mutants of Pseudomonas solanacearum unable to induce a hypersensitive reaction. Proc 4th Interactional Conference on Plant Pathogenic Bacteria. Station de pathologie végétale INRA, 49000 Beaucouzé, France, pp 823–833
Murai N, Skoog F, Doyle ME, Hanson R (1980) Relationships between cytokinin production, presence of plasmids, and fasciation caused by strains of Corynebacterium fascians. Proc Natl Acad Sci USA 77:619–623
Piwowarski JM, Shaw P (1982) Characterization of plasmids from plant pathogenic Pseudomonads. Plasmid 7:85–94
Rosenberg C, Casse-Delbart F, Dusha I, David M, Boucher C (1982) Megaplasmids in the plant associated Rhizobium meliloti and Pseudomonas solanacearum. J Bacteriol 150:402–406
Sequeira L, Kelman A (1961) The accumulation of growth substances in plant infected by Pseudomonas solanacearum. Phytopathology 52:439–448
Sparks RB, Lacy GH (1980) Purification and characterization of cryptic plasmids pLS1 and pLS2 from Erwinia chrysanthemi. Phytopathology 70:369–372
Staskawicz BJ, Dahlbeck D, Miller J, Damm D (1983) Molecular analysis of virulence genes in Pseudomonas solanacearum. In: Pülher A (ed) Molecular genetics of the bacteria-plant interaction. Springer, Berlin Heidelberg New York, pp 345–352
Van Gijsegem F, Toussaint A, Schoonejans E (1985) In vivo cloning of the pectate lyase and cellulase genes of Erwinia chrysanthemi. EMBO J 4:787–792
Van Larebecke N, Engler G, Holster M, Van den Elsacker S, Zaenen I, Schilperoot RA, Schell J (1974) Large plasmid in Agrobacterium tumefaciens essential for crown-gall inducing ability. Nature 252:169–170
White FF, Neser EW (1980) Hairy root: plasmid encodes virulence trait in Agrobacterium rhizogenes. J Bacteriol 141:1134–1141
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Communicated by J. Schell
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Boucher, C., Martinel, A., Barberis, P. et al. Virulence genes are carried by a megaplasmid of the plant pathogen Pseudomonas solanacearum . Molec. Gen. Genet. 205, 270–275 (1986). https://doi.org/10.1007/BF00430438
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DOI: https://doi.org/10.1007/BF00430438