Summary
Recombinant DNA techniques allow rapid construction and characterization of tools for genetic dissection of essential genes. In this report, we describe construction of a set of deletions in rpoD, the gene which encodes the sigma subunit of E. coli RNA polymerase. We used this deletion set to identify a region of the sigma polypeptide which is dispensable for its function.
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Hu, J.C., Gross, C.A. Marker rescue with plasmids bearing deletions in rpoD identifies a dispensable part of E. coli sigma factor. Molec. Gen. Genet. 191, 492–498 (1983). https://doi.org/10.1007/BF00425768
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DOI: https://doi.org/10.1007/BF00425768