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Effect of insulin-like growth factor I (IGF-I) administration on the healing of colonic anastomoses in rats

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Abstract

Background. The gastrointestinal tract is highly responsive to the trophic effect of growth hormone (GH). GH stimulates the healing of colonic anastomoses either directly or through insulin-like growth factor I (IGF-I) since specific GH receptor as well as IGF-I receptor have been demonstrated in colon. Aim. To determine whether exogeneous treatment with IGF-I could stimulate the healing of left colonic anastomoses in rats. Methods. After colonic anastomotic operations adult rats were randomised to treatment with either IGF-I (500 μg per day) or vehicle (controls). Anastomotic breaking strength and collagen deposition were determined at day 3 after surgery. Results. IGF-I treatment increased the anastomotic collagen content by 23% compared with controls. This resulted in a lower extensibility of the anastomosis (P<0.05), whereas the anastomotic breaking strength did not differ between groups. The treatment resulted in a 3 fold increase in serum IGF-I of IGF-I treated rats, compared to controls. The postoperative body weight increased by 5% in the IGF-I rats from day 0 to day 3, while the control group had a weight loss of 2% in the same peiod (P<0.001). Conclusion. Treatment of colon-operated rats with IGF-I increased the postoperative body weight and stimulates the collagen deposition of left colonic anastomoses, whereas the anastomotic strength may be unaffected by IGF-I treatment.

Résumé

Le tractus gastro-intestinal est très sensible aux effets trophiques de l'hormone de croissance (GH). GH stimule la guérison des anastomoses coliques soit directement, soit par l'intermédiaire d'un insulin-like facteur de croissance 1 (IGF-1) étant donné que l'on a pu mettre en évidence au niveau du côlon des récepteurs spécifiques pour GH de même que des récepteurs pour IGF-1. Le but de ce travail est de déterminer si un traitement exogène avec IGF-1 peut stimuler la guérison d'anastomose du côlon gauche chez le rat. Après résection-anastomose, des rats adultes ont été randomisés soit pour recevoir IGF-1 à omme groupe-contrôle. La résistance à la rupture de l'anastomose et le dépôt de collagène ont été déterminés trois jours après la chirurgie. Le traitement par IGF-1 augmente la teneur de collagène de l'anastomose de 23% comparativement au groupe de contrôle. Ceci résult dans une baisse de l'extensibilité de l'anastomose (P<0,05) alors que la résistance à la rupture de l'anatomose ne diffère pas entre les deux groupes. Le traitement a entraîné une augmentation par trois du taux d'IGF-1 en comparaison au groupe de contrôle. Le poids corporel augmente de 5% chez les rats traités par IGF-1 entre le jour 0 et le jour 3 alors que les animaux de contrôle ont une perte de poids de 2% durant la même période (P<0,001). Le traitement avec IGF-1 de rats opérés du côlon augmente le poids corporel et stimule la position de collagène au niveau de l'anastomose colique gauche alors que la résistance mécanique n'est pas modifiée par le traitement avec de l'IGF-1.

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Authors and Affiliations

  1. Department of Surgery L, University Hospital of Aarhus, Aarhus, Denmark

    T. Ingemann Petersen, P. Kissmeyer-Nielsen, A. flyvbjerg, S. Laurberg & H. Christensen

  2. Institute of Experimental Clinical Research, University Hospital of Aarhus, Aarhus, Denmark

    T. Ingemann Petersen, P. Kissmeyer-Nielsen, A. flyvbjerg, S. Laurberg & H. Christensen

  3. Surgical Research Unit, Department of Surgery L, University Hospital of Aarhus, Amtssygehuset, DK-8000, Aarhus C, Denmark

    P. Kissmeyer-Nielsen

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  1. T. Ingemann Petersen
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  3. A. flyvbjerg
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  4. S. Laurberg
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  5. H. Christensen
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Petersen, T.I., Kissmeyer-Nielsen, P., flyvbjerg, A. et al. Effect of insulin-like growth factor I (IGF-I) administration on the healing of colonic anastomoses in rats. Int J Colorect Dis 11, 19–24 (1996). https://doi.org/10.1007/BF00418850

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