Abstract
1. Chlorobium limicola forma sp. thiosulfatophilum was cultivated at 22 and 22000 lux. 2. The content of bchl d on a protein basis in the low light intensity cultures was about twice that of the high light intensity cultures. 3. After growth at 22 lux the red bchl d peak was at c. 743 nm, while at the higher intensity this peak was at c. 732 nm. 4. Electron microscopy of thin sections of Chlorobium revealed that vesicle size was greater at the low light intensity than at the high. 5. This was confirmed by sucrose density gradient centrifugation of differentially 14C-labelled vesicles from cultures grown at the two intensities. 6. The optimum temperature for growth was about 35°C. Incubation at the optimum temperature was particularly beneficial at high light intensity.
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Abbreviations
- bchl:
-
bacteriochlorophyll
References
Cohen-Bazire, G., Pfennig, N., Kunisawa, R.: The fine structure of green bacteria. J. Cell Biol. 22, 207–225 (1964)
Cruden, D. L., Stanier, R. Y.: The characterization of chlorobium vesicles and membranes isolated from green bacteria. Arch. Mikrobiol. 72, 115–134 (1970)
Ruller, R. C., Boyce, C.: The association of the photochemical reaction centre and the cytoplasmic membrane of the green bacteria. Paper presented at the Second International Symposium on Photosynthetic Procaryotes, Dundee, Aug. 22–28 (1976)
Holt, S. C., Conti, S. F., Fuller, R. C.: Photosynthesic apparatus in the green bacterium Chloropseudomonas ethylicum. J. Bacteriol. 91, 311–323 (1966a)
Holt, S. C., Conti, S. F., Fuller, R. C.: Effect of light intensity on the formation of the photochemical apparatus in the green bacterium Chloropseudomonas ethylicum. J. Bacteriol. 91, 349–355 (1966b)
Holt, S. C., Marr, A. G.: Effect of light intensity on the formation of intracytoplasmic membrane in Rhodospirillum rubrum. J. Bacteriol. 89, 1421–1429 (1965)
Liaaen Jensen, S., Hegge, E., Jackman, L. M.: Bacterial carotenoids. XVII. The carotenoids of photosynthetic green bacteria. Acta Chem. Scand. 18, 1703–1718 (1964)
Lowry, O. H., Rosebrough, M. J., Farr, A.L., Randall, R. J.: Protein measurements with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951)
Oelze, J.: Early formation of intracytoplasmic membranes in Rhodospirillum rubrum. Biochim. biophys. Acta 436, 95–100 (1976)
Olson, J. M., Giddings, T. H., Shaw, E. K.: An enriched reaction center preparation from green photosynthetic bacteria. Biochim. biophys. Acta 449, 197–208 (1976)
Pierson, B. K., Castenholz, R. W.: A phototrophic gliding filamentous bacterium of hot springs. Chloroflexus aurantiacus gen. and sp. nov. Arch. Microbiol. 100, 5–24 (1974)
Pfennig, N.: Eine vollsynthetische Nährlösung zur selektiven Anreicherung einiger Schwefelpurpurbakterien. Naturwissenschaften 48, 136 (1961)
Sirevåg, R.: Further studies on carbon dioxide fixation in Chlorobium. Arch. Microbiol. 98, 3–18 (1974)
Sirevåg, R.: Photoassimilation of acetate and metabolism of carbohydrate in Chlorobium thiosulfatophilum. Arch. Microbiol. 104, 105–111 (1975)
Stanier, R. Y., Smith, J. H. C.: The chlorophylls of green bacteria. Biochim. biophys. Acta 41, 478–484 (1960)
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Broch-Due, M., Ormerod, J.G. & Fjerdingen, B.S. Effect of light internsity on vesicle formation in Chlorobium . Arch. Microbiol. 116, 269–274 (1978). https://doi.org/10.1007/BF00417850
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DOI: https://doi.org/10.1007/BF00417850