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Abstract

Experimental studies of ocular inflammation induced by endotoxin have been performed almost exclusively in rabbits. Although the rat has been considered refractory to both the systemic and local effects of endotoxin, the present study has shown that intravitreal injection of endotoxin evoked a characteristic acute inflammatory response which was reproducible and dose-dependent. In addition, a consensual ocular response in the control eye occurred which was less severe but also dose-dependent. Preliminary investigations support the view that consensual responses of this nature may be mediated by a nervous reflex arc mechanism. Sequential histological analysis revealed a marked polymorphonuclear cell infiltration of most ocular tissues in the early stages, while mononuclear cells predominated after 3 days. Widespread intravascular microthrombi were also observed, particularly in the ciliary vessels. Recovery, however, was usually complete by 7 days.

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References

  • Apitz, K.: A study of the generalized Shwartzman phenomenon. J. Immunol. 29, 255–264 (1935)

    Google Scholar 

  • Ayo, C.: A toxic ocular action. New property of Shwartzman toxins. J. Immunol. 46, 113–132 (1943)

    Google Scholar 

  • Behrens, M., Manski, W.: Immunogenic uveitis in inbred rats. Ophthalmol. Res. 5, 31–40 (1973)

    Google Scholar 

  • Beitch, S.R., Eakins, K.E.: Effects of prostaglandins on the intraocular pressure of the rabbit. Br. J. Pharmacol. 37, 158–167 (1969)

    Google Scholar 

  • Bito, L.Z.: The effects of experimental uveitis on anterior uveal prostaglandin transport and aqueous humor composition. Invest. Ophthalmol. 13, 959–966 (1974)

    Google Scholar 

  • Bito, L.Z.: Inflammatory effects of endotoxin-like contaminants in commonly used protein preparations. Science 196, 83–85 (1977)

    Google Scholar 

  • Butler, J.M., Unger, W.G., Hammond, B.R.: Sensory mediation of the ocular response to neutral formaldehyde. Exp. Eye Res. 28, 577–589 (1979)

    Google Scholar 

  • Butler, J.M.: Substance P as a mediator of the ocular injury response. (Abstr) Association for Eye Research, 20th Meeting, Jouy-en-Josas, France, 1979

    Google Scholar 

  • Chien, S., Sinclair, D.G., Dellenback, R.J.: Effect of endotoxin on capillary permeability to macromolecules. Am. J. Physiol. 207, 518–522 (1964)

    Google Scholar 

  • Chando, J.W.: Affinity of endotoxin for membranes. J. Infect. Dis. 128, 5197–5201 (1973)

    Google Scholar 

  • Guillery, F.: Ueber Ferment-Wirkung des Auges und ihre Beziehungen zu sympathischer Ophthalmie. Arch. Augenheilk. 68, 242–289 (1911)

    Google Scholar 

  • Horn, R.G.: Evidence for participation of granulocytes in the pathogenesis of the generalised Shwartzmann reaction: A review. J. Infect. Dis. 128, 5134–5142 (1973)

    Google Scholar 

  • Howes, E.L., McKay, D.G.: Effect of cervical sympathectomy on the ocular response to systemic endotoxin. Proc. Soc. Exp. Biol. Med. 139, 839–844 (1972)

    Google Scholar 

  • Howes, E.L., McKay, D.G.: Ocular changes in the generalised Shwartzmann reaction. Arch. Ophthalmol. 90, 218–224 (1973)

    Google Scholar 

  • Howes, E.L., McKay, D.G., Margaretten, W.: The participation of the platelet in the vascular response to endotoxaemia in the rabbit eye. Am. J. Pathol. 70, 25–37 (1973)

    Google Scholar 

  • Kaley, G., Demopoulos, B.S., Zweifach, H.: Occlusive vascular lesions induced by bacterial endotoxin in kidneys of pregnant rats. Proc. Soc. Exp. Biol. Med. 109, 456–460 (1962)

    Google Scholar 

  • Kottow, M.H., Seligman, L.J.: Consensual reactions to anterior chamber paracentesis in the rabbit. Am. J. Ophthalmol. 85, 392–399 (1978)

    Google Scholar 

  • Leplat, G.: Etude de quelques reactions provoquees dans les yeux par une contusion oculaire unilaterale. Ann. Oculist. 161, 87–106 (1924)

    Google Scholar 

  • Levene, R.Z.: General and ocular effects of endotoxin. Surv. Ophthalmol. 4, 581–592 (1959)

    Google Scholar 

  • Levene, R.Z., Breinin, C.M.: Ocular effects of endotoxin. Arch. Ophthalmol. 88, 568–577 (1959)

    Google Scholar 

  • Magitot, A.: Sur le tension oculaire et quelques-unes de ses modifications experimentales. Ann. Oculist. 160, 81–105 (1923)

    Google Scholar 

  • Morrison, D.C., Cochrane, C.G.: Direct evidence for Hageman factor activation by bacterial lipopolysaccharides. J. Exp. Med. 140, 797–811 (1974)

    Google Scholar 

  • Nowotny, A.: Molecular aspects of endotoxic reactions. Bact. Revs. 33, 72–98 (1969)

    Google Scholar 

  • Pappas, G.D., Smelser, G.K.: Studies on the ciliary epithelium and the zonule. Electron microscopic observations on changes induced by alteration of normal aqueous humor formation in the rabbit. Am. J. Ophthalmol. 46, 299–317 (1959)

    Google Scholar 

  • Perkins, E.S.: Influence of the fifth cranial nerve on the intra-ocular pressure of the rabbit. Br. J. Pharmacol. 37, 158–167 (1969)

    Google Scholar 

  • Rietschel, E.T.: Chemical structure and biological activity of endotoxins (lipopolysaccharides) and lipid A. Naunyn Schmiedebergs Arch. Pharmacol. 287, 74–84 (1975)

    Google Scholar 

  • Segawa, K., Smelser, G.K.: Electron microscopy of experimental uveitis. Invest. Ophthalmol. 8, 497–520 (1969)

    Google Scholar 

  • Taichman, M.S., Uriuhara, T., Movat, H.Z.: Ultrastructural alterations in the local Shwartzman reaction. Lab. Invest. 14, 2160–2176 (1966)

    Google Scholar 

  • Theiss, W., Hilgard, P., Hayes, H.: Induction of disseminated intravascular coagulation by endotoxin and saline loading in rats. Fibrin deposition and removal. Thromb. Res. 7, 47–58 (1975)

    Google Scholar 

  • Tilgner, S., Henyrel, E., Meyer, W., Schumann, M.: Ophthalmologische Untersuchungen über die Adjuvanskrankheit der Ratte. Klinische und histologische Befunde. Albrecht v. Graefe's Arch. klin. exp. Ophthalmol. 183, 348–362, 1972, a.

    Google Scholar 

  • Tilgner, S., Henyrel, E., Meyer, W., Schumann, M.: Ophthalmologische Untersuchungen über die Adjuvanskrankheit der Ratte. Quantitative Daten zum klinischen Bild unter besonderer Berücksichtigung der Residive. Albrecht v. Graefe's Arch. klin. exp. Ophthalmol. 184, 155–166, 1972, b.

    Google Scholar 

  • Waksman, B.H., Bullinton, S.J.: Studies of arthritis and other lesions induced in rats by injection of mycobacterial adjuvant. Lesions of the eye. Arch. Ophthalmol. 64, 752–762 (1960)

    Google Scholar 

  • Zygulska-Mach, H., Mach, Z.: Über die Einwirkung von Gammastrahlen auf den Gehalt des Glutathions unter der Ascorbinsäure in Kaninchenlinsen. Klin. Mbl. Augenheil 166, 50–55 (1975)

    Google Scholar 

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This study was supported by National Institute of Health Grants EY-02648-01 and EY-01747 from the National Eye Institute

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Forrester, J.V., Worgul, B.V. & Merriam, G.R. Endotoxin-induced uveitis in the rat. Albrecht von Graefes Arch. Klin. Ophthalmol. 213, 221–233 (1980). https://doi.org/10.1007/BF00417543

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