Abstract
Superoxide radicals were investigated as to their capability of depolymerizing the hyaluronic acid of the bovine vitreous body. Using viscometry it was found that O2 radicals, generated by the hypoxanthine/xanthine oxidase method or the combination of NADH and phenazine methosulphate, degraded hyaluronic acid. This reaction was suppressed by superoxide dismutase, catalase, and peroxidase. In contrast, the depolymerization of hyaluronic acid by oxidation-reduction systems like ascorbic acid or ferrous ions was abolished by catalase and peroxidase while superoxide dismutase showed no effect.
Zusammenfassung
Es wurde die Fähigkeit von Superoxidradikalen untersucht, die Hyaluronsäure des Rinderglaskörpers zu depolymerisieren. Mittels Viskosimetrie wurde festgestellt, daß O2 Radikale, die entweder aus einem Gemisch von Hypoxanthin und Xanthin-Oxidase oder aus NADH und Phenazinmethosulfat erzeugt wurden, die Hyaluronsäure abbauten. Diese Reaktion wurde von Superoxiddismutase, Katalase und Peroxidase unterdrückt. Im Gegensatz dazu wurde die Depolymerisation der Hyaluronsäure durch Oxidation-Reduktion Systeme, wie Ascorbinsäure oder Fe2+-Ionen, nur von Katalase und Peroxidase verhindert, während die Superoxiddismutase keine Wirkung zeigte.
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Hofmann, H., Schmut, O. The inability of superoxide dismutase to inhibit the depolymerization of hyaluronic acid by ferrous ions and ascorbate. Albrecht von Graefes Arch. Klin. Ophthalmol. 214, 181–185 (1980). https://doi.org/10.1007/BF00414560
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DOI: https://doi.org/10.1007/BF00414560