Summary
CO2 curves of photosynthesis and activities of the four C4 enzymes and Ribulose bisphosphate carboxylase (RUBPc) were compared in two populations of the C4 grass Echinochloa crus-galli from contrasting thermal environments (Québec and Mississippi). Analyses were conducted both before and after 14 h of chilling at 7°C under high light conditions. This comparison provides the opportunity to assess which steps of the C4 pathway are more susceptible to become limiting at low temperatures. Both populations maintained, after chilling, a pattern of CO2 fixation typical of C4 plants with photosynthesis saturating at low external CO2 concentrations. However, the chilling treatment led to reductions in carbon uptake and in the activities of the C4 enzymes. RUBPc activity was not significantly affected by chilling. Reductions in photosynthesis and in C4 enzyme activities following the chilling treatment were significantly larger for plants of the Mississippi population. The enzyme data suggest that two steps of the C4 pathway, NADP+-malate dehydrogenase and pyruvate Pi dikinase, are likely to be associated with the reduction of CO2 uptake in C4 plants under cool conditions. When the experiment was replicated under enriched atmospheric CO2 (675 μl l-1 CO2), similar differences were observed between the two populations. CO2 enrichment resulted in an increase of activity for phospho-enol-pyruvate carboxylase and NADP+-malate dehydrogenase while activities of phospho-enol-pyruvate carboxylase and NADP+-malic enzyme were less reduced following chilling. Such an interaction was not observed for gas exchange parameters but net photosynthesis was lower when plants were grown under enriched CO2.
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References
Bruinsma J (1963) The quantitative analysis of chlorophylls a and b in plant extracts. Photochem Photobiol 2:241–249
Caldwell MM, Osmond CB, Nott DL (1977) C4 pathway photosynthesis at low temperature in cold-tolerant Atriplex species. Plant Physiol 60:157–164
Edwards GE, Ku MSB, Hatch MD (1982) Photosynthesis in Pamicum milioides, a species with reduced photorespiration. Plant Cell Physiol 23:1185–1195
Farquhar GD, Sharkey TD (1982) Stomatal conductance and photosynthesis. Ann Rev Plant Physiol 33:317–345
Gutierrez M, Gracen VE, Edwards GE (1974) Biochemical and cytological relationships in C4 plants. Planta 119:279–300
Hatch MD (1979) Regulation of C4 photosynthesis: Factors affecting cold-mediated inactivation and reactivation of pyruvate, Pi dikinase. Aust J Plant Physiol 6:607–619
Hatch MD, Osmond CB (1976) Compartmentation and transport in C4 photosynthesis. In: Pirson A, Zimmermann MH (eds) Encyclopedia of plant physiology. New series, vol 3. Springer, Berlin Heidelberg New York, pp 144–184
Long SP (1983) C4 photosynthesis at low temperature. Plant Cell Environ 6:345–363
Long SP, East TM, Baker NR (1983) Chilling damage to photosynthesis in young Zea mays I. Effects of light and temperature variation on photosynthetic CO2 assimilation. J Exp Bot 34:177–188
Lorimer GH, Badger MR, Andrews TJ (1977) D-Ribulose-1,5-bisphosphate carboxylase oxygenase: Improved methods for the activation and assay of catalytic activities. Anal Biochem 78:66–74
Morisson JIL, Gifford RM (1983) Stomatal sensitivity to CO2 and humidity. A comparison of two C3 and two C4 grass species. Plant Physiol 71:789–796
Nakamoto H, Edwards GE (1983) Influence of oxygen and temperature on the dark inactivation of pyruvate orthophosphate dikinase and NADP-malate dehydrogenase in maize. Plant Physiol 71:568–573
Pearcy RW, Ehleringer J (1984) Comparative ecophysiology of C3 and C4 plants. Plant Cell Environ 7:1–13
Potvin C (1985a) Biomass allocation and phenological differences among southern and northern populations of the C4 grass Echinochloa crus-galli. J Ecol (in press)
Potvin C (1985b) Amelioration of chilling effects by CO2 enrichment. Physiol Veg 23:345–352
Potvin C (1985c) Responses of two C4 grasses to CO2 enrichment and low temperature: implications for the biogeographical distribution of C4 plants. Ph D Thesis, Duke University, Univ Microfilm Inter, Ann Arbor, Mich, Publication No: 8523043 (Diss Abst 4611)
Potvin C, Strain BR (1985) Photosynthetic response to growth temperature and CO2 enrichment in two species of C4 grasses. Can J Bot 63:483–487
Rogers HH, Thomas JF, Bingham GE (1983) Response of agronomic and forest species to elevated atmospheric carbon dioxide. Science 220:428–429
Simon J-P, Potvin C, Blanchard M-H (1983) Thermal adaptation and acclimation of higher plants at the enzyme level: kinetic properties of NAD malate dehydrogenase and glutamate oxaloacetate transaminase in two genotypes of Arabidopsis thaliana (Cruciferae). Oecologia (Berlin) 60:143–148
Simon J-P, Potvin C, Strain BR (1984) Effects of temperature and CO2 enrichment on kinetic properties of phospho-enol-pyruvate carboxylase in two ecotypes of Echinochloa crus-galli (L) Beauv, a C4 weed grass species. Oecologia (Berlin) 63:145–152
Sionit N, Strain BR, Beckford HA (1981) Environmental controls on the growth and yield of okra I. Effect of temperature and CO2 enrichment at cool temperature. Crop Sci 21:885–888
Smith AM, Woolhouse HW, Jones DA (1982) Photosynthetic carbon metabolism of the cool-temperate C4 grass Spartina anglica Hubb. Planta 156:441–448
Sugiyama T, Boku K (1976) Differing sensitivity of pyruvate orthophosphate dikinase to low temperature in maize cultivars. Plant Cell Physiol 17:851–854
Sugiyama T, Schmitt MR, Ku SB, Edwards GE (1979) Differences in cold lability of pyruvate, Pi dikinase among C4 species. Plant Cell Physiol 20:965–971
Taylor AO, Slack CR, McPherson HG (1974) Plant under climatic stress. VI. Chilling and light effects on photosynthetic enzymes of sorghum and maize. Plant Physiol 54:696–701
Thomas SM, Long SP (1978) C4 photosynthesis in Spartina townsendii at low and high temperatures. Planta 142:171–174
Usuda U, Ku MSB, Edwards GE (1984) Rates of photosynthesis relative to activity of photosynthetic enzymes, chlorophyll and soluble protein content among ten C4 species. Aust J Plant Physiol 11:509–517
Usuda H, Ku MSB, Edwards GE (1985) Influence of light intensity during growth on photosynthesis and activity of several key photosynthetic enzymes in a C4 plant (Zea mays). Physiol Plant 63:65–70
Van Bavel MCH (1974) Antitranspirant action of carbon dioxide on intact Sorghum plants. Crop Sci 14:208–212
Wardlaw IF (1982) Assimilate movement in Lolium and Sorghum leaves. III. Carbon dioxide concentration effects on the metabolism and translocation of photosynthate. Aust J Plant Physiol 9:705–713
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Potvin, C., Simon, JP. & Strain, B.R. Effect of low temperature on the photosynthetic metabolism of the C4 grass Echinochloa crus-galli . Oecologia 69, 499–506 (1986). https://doi.org/10.1007/BF00410354
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DOI: https://doi.org/10.1007/BF00410354