Abstract
The mechanism of lysis of Eubacterium alactolyticum cell walls by Streptomyces albus G enzyme was studied. The analysis of the peptide terminal groups and peptide subunits isolated from the cell wall digest, released during solubilization of the cell walls, revealed that lytic action of S. albus G enzyme was mainly due to D-alanyl-A2pm endopeptidase, N-acetylmuramyl-L-alanine amidase, N-acetylmuramidase and N-acetylglucosaminidase. E. alactolyticum cell wall peptidoglycan is composed mainly of glucosamine, muramic acid, D-glutamic acid, L- and D-alanine, meso-diaminopimelic acid and glycine. The peptide subunit consists of L-alanyl-D-glutamyl-meso-A2pm-D-alanine. D-Alanine is connected directly with the amino group of the meso-A2pm residue of another peptide subunit. All of the L-amino groups of meso-diaminopimelic acid are involved in cross-linking.
The possible structure of the peptide moiety of E. alactolyticum cell wall peptidoglycan is presented.
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References
Ashwell G (1957) Colorimetric analysis of sugars. Meth Enzymol 3:73–105
Bricas E, Ghuysen J-M, Dezelee P (1967) The cell wall peptidoglycan of Bacillus megaterium M. I. Studies on the stereochemistry of α,α′-diaminopimelic acid. Biochemistry 6:2598–2607
Fukuhara H, Umemoto T, Sagawa H, Kato K, Kotani S (1983) Purification and quantitative chemical analysis of cell wall peptidoglycans of Leptotrichia buccalis. Infect Immun 39:132–136
Ghuysen J-M, Dierickx L, Leyh-Bouille M, Strominger JL, Bricas E, Nicot C (1965) Biochemistry 4:2237–2244
Ghuysen J-M, Tipper DJ, Strominger JL (1966) Enzymes that degrade bacterial cell walls. Meth Enzymol 8:685–699
Guinand M, Ghuysen J-M, Schleifer KH, Kandler O (1969) The peptidoglycan in walls of Butyribacterium rettgeri. Biochemistry 8:200–207
Hamada S, Kotani S, Kato K (1968) Studies on cell walls of group A Streptococcus pyogenes, Type 12. 1. Mechanism of lysis by the L-11 enzyme and possible structure of a peptide moiety of the peptidoglycan. Biken J 11:293–311
Jürgens UJ, Meissner J, Fischer U, König WA, Weckesser J (1987) Ornithin as a constituent of the peptidoglycan of Chloroflexus aurantiacus, diaminopimelic acid in that of Chlorobium vibrioforme f. thiosulfatophilum. Arch Microbiol 148:72–76
Kato K, Iwata S, Suginaka H, Namba K, Kotani S, Tamura T (1976) Chemical structure of the peptidoglycan of Vibrio parahaemolyticus A55 with special reference to the extent of interpeptide cross-linking. Biken J 19:139–150
Kato K, Umemoto T, Fukuhara H, Sagawa H, Kotani S (1981) Variation of dibasic amino acid in the cell wall peptidoglycan of bacteria of genus Fusobacterium. FEMS Microbiol Lett 10:81–85
Lowry OH, Roberts NR, Leiner KY, Wu ML, Farr AL (1954) The quantitative histochemistry of brain. J Biol Chem 207:1–17
Park JT, Johnson MJ (1949) A submicrodetermination of glucose. J Biol Chem 181:149–151
Schleifer KH, Kandler O (1972) Peptidoglycan types of bacterial cell walls and their taxonomic implication. Bacteriol Rev 36:407–477
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Severin, A.I., Kokeguchi, S. & Kato, K. Chemical composition of Eubacterium alactolyticum cell wall peptidoglycan. Arch. Microbiol. 151, 348–352 (1989). https://doi.org/10.1007/BF00406563
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DOI: https://doi.org/10.1007/BF00406563