Abstract
Total glutathione content, glutathione peroxidase, glutathione transferase and glutathione reductase activities have been measured in 12 species of yeasts. All the strains tested contained glutathione, though in different amounts, as well as the above mentioned enzymes. To discriminate between the selenium-dependent and the selenium-independent form, glutathione peroxidase activity has been measured with both H2O2 and cumene hydroperoxide. Rhodotorula glutinis appeared to be the only strain in which the selenium-dependent form was not found, but this yeast exhibited the highest level of selenium-independent glutathione peroxidase activity as compared to the other strains.
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Aisaka, K, Uwajima, T & Tereda, O (1983) Production of glutathione peroxidase by Mucor hiemalis. Agric. Biol. Chem. 47: 1269–1273
Akerboom, TPM & Sies, H (1981) Assay of glutat mixed disulfide in biological samples. In: Jacoby, WB (Ed) Methods in Enzymology Vol. 77 (pp 373–382) Academic Press, New York
Balloni, W, Florenzano, G, Mazza, G & Polsinelli, M (1987) Candida amidevorans sp. nov., a new ascomycetous anamorph yeast from soil. Antoine van Leeuwenhoek 53: 99–106
Clark, AG & Dauterman, WC (1982) The characterization by affinity chromatography of glutathione S-transferase from different strains of house fly. Pest. Biochem. Physiol. 17: 307–314
Di Ilio, C, Polidoro, G, Arduini, A, Muccini, A & Federici, G (1983) Glutathione peroxidase, glutathione reductase, glutathione S-transferase and gamma-glutamyltranspeptidase activities in human early pregnancy placenta. Biochem. Med. 29: 143–148
Flohè, L, Gunzler, WA & Loshen, G (1980) The glutathione peroxidase reaction, a key to understand the selenium requirement of mammals. In: Kharash, N (Ed) Trace Metals in Health and Disease (pp 263–286) Raven Press, New York
Habig, WH, Pabst, MJ & Jacoby, YB (1974) Glutathione S-transferase: the first enzymatyc step in mercapturic acid formation. J. Biol. Chem. 243: 809–814
Hazelton, GA & Lang, CA (1985) Glutathione peroxidase and reductase activity in the aging mouse. Mech. Ageing Dev. 29: 71–81
Igarashi, T, Satho, T, Ueno, K & Kitagawa, H (1983) Sex-related difference in the hepatic glutathione level and related activities in rat. J. Biochem. 93: 33–36
Jacoby, WB (1978) The glutathione S-transferase: a group of multifunctional detoxicant proteins. Adv. Enzymol. 46: 383–414
James, MO, Bowen, ER, Dansette, PM & Bend, JR (1979) Epoxide hydrase and glutathione S-transferase activities with alkene and arene oxides in several marine species. Chem. Biol. Interact. 25: 321–325
Kistler, M, Summer, K-H & Eckardt, F (1986) Isolation of glutathione-deficient mutants of the yeast Saccharomyces cerevisiae. Mutation Research 173: 117–120
Lau, EP, Niswander, L, Watson, D & Fall, R (1980) Glutathione S-transferase in a variety of microorganisms. Chemosphere 9: 565–569
Lawerence, RA & Burk, RF (1976) Glutathione peroxidase activity in selenium-deficient rat liver. Biochem. Biophys. Res. Commun. 71: 952–958
Lawerence, RA & Burk, RF (1978) Species, tissue and subcellular distribution of non Se-dependent glutathione peroxidase activity. J. Nutr. 108: 211–215
Lowry, OH, Rosenbrough, NJ Farra, AL & Randall, RJ (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 87–98
Mannervik, B (1985) The isozymes of glutathione transferase. Adv. Enzymol. Relat. Areas Mol. Biol. 57: 357–417
Mavis, RD & Stellwagen, E (1968) Purification and subunit structure of glutathione reductase from baker's yeast. J. Biol. Chem. 243: 809–814
Meister A & Anderson MZ (1983) Glutathione. In Ann. Rev. Biochem. Vol. 52, pp 711–760
Mozer, TJ, Tiemeier, DC & Jaworski, EG (1983) Purification and characterization of corn glutathione S-transferase. Biochemestry 22: 1068–1072
Mukhtar, H, Zoetemelk, EM, Baars, AJ, Blankenstein-Wijnen, MM, Khan, PM & Breimer, DD (1981) Glutathione S-transferase activity in human fetal and adult tissues. Pharmacology 22: 322–329
Pan, JG & Rheee, JS (1986) Kinetic and energetic analyses of lipid accumulation in batch culture of Rhodotorula glutinis. J. Ferment. Technol. 64: 557–560
Penninckx, MJ & Jaspers, CJ (1982) On the role of glutathione in microorganisms. Bull. Inst. Pasteur 80: 291–301
Prohaska, JR (1980) The glutathione peroxidase activity of glutathione S-transferase. Biochim. Biophys. Acta 611: 87–98
Rennenberg, H (1982) Glutathione metabolism and biological roles in higher plants. Phytochemestry 21: 2771–2781
Shishido, T (1981) Glutathione S-transferase from Escherichia coli. Agric. Biol. Chem. 45: 2951–2953
Smith, J & Shrift, A (1978) Phylogenetic distribution of glutathione peroxidase. Comp. Biochem. Physiol. 63B: 39–44
Summer, K-H, Goggelman, W & Greim, H (1980) Glutathione and glutathione S-transferase in the Salmonella mammalian-microsome mutagenicity test. Mutation Res. 70: 269–278
Tappel, ME, Chaudiere, J & Tappel, AL (1982) Glutathione peroxidase activity of aminal tissues. Comp. Biochem. Physiol. 73B: 945–949
Yawetz, A & Agosin, M (1981) Purification of glutathione S-transferase of Trypanosoma cruzi. Comp. Biochem. Physiol. 68/2B: 273–243
Wendel, A (1980) Glutathione peroxidase. In: Jakoby, WB (Ed)_Enzymatic Basis of Detoxication Vol. 1 (pp 333–348) Academic Press. New York
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Casalone, E., Di Ilio, C., Federici, G. et al. Glutathione and glutathione metabolizing enzymes in yeasts. Antonie van Leeuwenhoek 54, 367–375 (1988). https://doi.org/10.1007/BF00393527
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DOI: https://doi.org/10.1007/BF00393527