Abstract
Ammonia-treated bagasse with 80%(w/w) moisture content was subjected to mixed-culture solid-substrate fermentation (SSF) with Trichoderma reesei LM-UC4 and Aspergillus phoenicis QM 329, in flask or pot fermenters, for cellulase production. Significantly higher activities of all the enzymes of the cellulase complex were achieved in 4 days of mixed-culture SSF than in single-culture (T. reesei) SSF. The highest filter-paper-cellulase and β-glucosidase activities seen in mixed-culture SSF were 18.7 and 38.6 IU/g dry wt, respectively, representing approx. 3- and 6-fold increases over the activities attained in single-culture SSF. The mixed-culture SSF process also converted about 46% of the cellulose and hemicellulose to reducing sugars and enriched the product with 13% fungal protein. The biomass productivity, 0.29 gl-1.h, and enzyme productivity, 28.0 IU I-1.h, were about twice as high in the mixed-culture than in the single-culture.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
AbdullahA.L., TengerdyR.P. & MurphyV.G. 1985 Optimization of solid substrate fermentation of wheat straw. Biotechnology and Bioengineering 27, 20–27.
DeschampsF., GiulianoC., AstherM., HuetM.C. & RoussosS. 1985 Cellulase production by Trichoderma harzianum in static and mixed solid-state fermentation reactors under nonaseptic conditions. Biotechnology and Bioengineering 27, 1385–1388.
DuffS.J.B. 1985 Cellulase and beta-glucosidase production by mixed culture of Trichoderma reesei Rut C30 and Aspergillus phoenicis. Biotechnology Letters 7, 185–190.
DuffS.J.B., CooperD.G. & FullerO.M. 1986 Evaluation of the hydrolytic potential of crude cellulase from mixed cultivation of Trichoderma reesei and Aspergillus phoenicis. Enzyme and Microbial Technology 8, 305–308.
DuffS.J.B., CooperD.G. & FullerO.M. 1987 Effect of media composition and growth conditions on production of cellulase and β-glucosidase by a mixed fungal fermentation. Enzyme and Microbial Technology 9, 47–52.
GoeringH.K. & VanSoestP.J. 1970 Forage Fiber Analysis, Agriculture Handbook, No. 379. Washington DC: Department of Agriculture.
Gutierrez-CorreaM. 1987 Bioconversion de residuos lignocelulosicos en etanol y proteina. In Ciencia y Tecnologia para el Desarrollo, pp. 81–95. Lima: CONCYTEC.
JulcaM. & Gutierrez-CorreaM. 1987 Produccion de cellulasas por hongos: obtencion de mutantes hiperceluloliticos. Boletin de Lima 54, 48–54.
LaukevicsJ.J., ApsiteA.F., ViestursU.E. & TengerdyR.P. 1984 Solid substrate fermentation of wheat straw to fungal protein. Biotechnology and Bioengineering 26, 1465–1474.
MadamwarD., PatelS. & ParikhH. 1989. Solid state fermentation for cellulases and β-glucosidase production by Aspergillus niger. Journal of Fermentation and Bioengineering 67, 424–426.
MandelsM., SternbergD. & AndreotiR.E. 1975 Growth and cellulase production by Trichoderma. In Proceedings of Symposium on Enzymatic Hydrolysis of Cellulose eds BaileyM., EnariT.M. & LinkoM. pp. 81–109. Helsinki: SITRA.
PandaT., BisariaV.S. & GhoseT.K. 1983 Studies on mixed fungal culture for cellulase and hemicellulase production. Part 1. Optimization of medium for the mixed culture of Trichoderma reesei D1-6 and Aspergillus wentii Pt 2804. Biotechnology Letters 5, 767–772.
Reese, E.T. & Mandels, M. 1966 β-Glucanases other than cellulases. Methods in Enzymology 8, 607–615.
SaddlerJ.N. 1982 Screening of highly cellulolytic fungi and the action of their cellulase enzyme systems. Enzyme and Microbial Technology 4, 414–418.
SaddlerJ.N., Mes-HartreeM., YuE.K.C. & BrownellH.H. 1983 Enzymatic hydrolysis of various pretreated lignocellulosic substrates and the fermentation of the liberated sugar to ethanol and butanediol. Biotechnology and Bioengineering Symposium 13, 225–238.
ShamalaT.R. & SreekantiahK.R. 1987 Successive cultivation of selected cellulolytic on rice straw and wheat bran for economic production of cellulases and D-xylanase. Enzyme and Microbial Technology 9, 97–101.
Sternberg, D., Vijayakumar, P. & Reese, E.T. 1977 β-Glucosidase: microbial production and effect on enzymatic hydrolysis of cellulose. Canadian Journal of Microbiology 23: 139–147.
TengerdyR.P. 1992 Solid state fermentation of lignocellulose. In Solid Substrate Cultivation, eds DoelleH.W., MitchellD.A. & RolzC.E. pp. 269–282. London: Elsevier Applied Science.
UlmerD.C., TengerdyR.P. & MurphyV.G. 1981 Solid state fermentation of steam treated feedlot waste fibers with Chaetomium cellulolyticum. Biotechnology and Bioengineering Symposium 11, 449–461.
Additional information
R. Dueñas is with the Departamento de Biologia, Universidad Nacional San Antonio Abad, Cusco, Peru. R. Tengerdy is with the Department of Microbiology, Colorado State University, Fort Collins, CO 80523, USA. M. Gutierrez-Correa is with the Laboratorio de Micologia y Biotecnologia, Universidad Nacional Agraria La Molina, Apdo. Postal 456, Lima 1. Peru;
Rights and permissions
About this article
Cite this article
Dueñas, R., Tengerdy, R.P. & Gutierrez-Correa, M. Cellulase production by mixed fungi in solid-substrate fermentation of bagasse. World Journal of Microbiology & Biotechnology 11, 333–337 (1995). https://doi.org/10.1007/BF00367112
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00367112