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Quantification of trehalose in biological samples with a conidial trehalase from the thermophilic fungus Humicola grisea var. thermoidea

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Abstract

The partially-purified, thermally-stable trehalase from conidia of Humicola grisea was highly specific for trehalose and was free of potentially interfering activities. The enzyme was fully stable when stored in solution at -15°C for at least 6 months. This preparation could be used to quantify trehalose from 0.05 to 1.25 μmol/ml either in carbohydrate mixtures or in complex biological materials.

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References

  • Araújo, P.S., Panek, A.C., Ferreira, R. & Panek, A.D. 1989 Determination of trehalose in biological samples by a simple and stable trehalase preparation. Analytical Biochemistry 176, 432–436.

    Google Scholar 

  • Brin, M. 1966 Transketolase: clinical aspects. Methods in Enzymology 9, 506–514.

    Google Scholar 

  • Curtois, J.E. & Demelier, J.F. 1966 Repartition de la trehalase chez l'homme et quelques mammifères. Bulletin de la Société de Chimie Biologique 48, 277–286.

    Google Scholar 

  • Hottiger, T., Schmutz, P.S. & Wiemken, A. 1987 Heat-induced accumulation and futile cycling of trehalose in Saccharomyces cerevisiae. Journal of Bacteriology 169, 5518–5522.

    Google Scholar 

  • Hugget, A.S.G. & Nixon, P.A. 1957 Enzymic determination of blood glucose. Biochemical Journal 66, P12.

    Google Scholar 

  • Kelly, P.J. & Catley, B.J. 1976 A purification of trehalase from Saccharomyces cerevisiae. Analytical Biochemistry 72, 353–358.

    Google Scholar 

  • Neves, M.J., Jorge, J.A., François, J.M. & Terenzi, H.F. 1991 Effects of heat shock on the level of trehalose and glycogen, and on the induction of thermotolerance in Neurospora crassa. FEBS Letters 283, 19–22.

    Google Scholar 

  • Peralta, R.M., Terenzi, H.F. & Jorge, J.A. 1990 β-d-Glycosidase activity of Humicola grisea: biochemical and kinetic characterization of a multifunctional enzyme. Biochimica et Biophysica Acta 1033, 243–249.

    Google Scholar 

  • Spiro, R.G. 1966 Analysis of sugars found in glycoproteins. Methods in Enzymology 18, 3–5.

    Google Scholar 

  • Thevelein, J.M. 1984 Regulation of trehalose mobilization in fungi. Microbiological Reviews 48, 42–59.

    Google Scholar 

  • Thevelein, J.M. 1988 Regulation of trehalase activity by phosphorylation-dephosphorylation during developmental transitions in fungi. Experimental Mycology 12, 1–12.

    Google Scholar 

  • Zimmermann, A.L.S., Terenzi, H.F. & Jorge, J.A. 1990 Purification and properties of an extracellular conidial trehalase from Humicola grisea var. thermoidea. Biochimica et Biophysica Acta 1036, 41–46.

    Google Scholar 

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M.J. Neves, H.F. Terenzi and J.A. Jorge are with Departmento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirao Preto, Universidade de São Paulo, 14040-901-Ribeirão Preto, São Paulo, Brazil; F.A. Leone is with Departamento de Quimica, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, 14040-901-Ribeirão Preto, São Paulo, Brazil.

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Neves, M.J., Terenzi, H.F., Leone, F.A. et al. Quantification of trehalose in biological samples with a conidial trehalase from the thermophilic fungus Humicola grisea var. thermoidea . World Journal of Microbiology & Biotechnology 10, 17–19 (1994). https://doi.org/10.1007/BF00357555

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  • DOI: https://doi.org/10.1007/BF00357555

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