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A novel transducing phage

Its role in recognition of a possible new host-controlled modification system in Pseudomonas aeruginosa

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Summary

Two newly isolated generalized transducing phages, F126 and F130, are in many respects similar to the previously described phage F116 of P. aeruginosa strain PAO. They also share with F116 the property of not responding to host-specific restriction in strain PAO. However, while the transduction ability of phages F116 and F130 is also inert to PAO-specific restriction, transduction mediated by phage F126 is 8–120 fold reduced in restrictive conditions. In experiments designed to explore the conditions necessary to obtain restriction in F126 transduction it was found that it differed from those previously known to specify host-controlled restriction in P. aeruginosa PAO (Rolfe and Holloway, 1968). These observations suggest that more than one independent restriction system operates in strain PAO.

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Communicated by W. Arber

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Krishnapillai, V. A novel transducing phage. Molec. Gen. Genetics 114, 134–143 (1972). https://doi.org/10.1007/BF00332784

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  • DOI: https://doi.org/10.1007/BF00332784

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