Summary
We present evidence that the Escherichia coli DNA binding protein, IHF, plays an important role in conjugal transfer of the plasmid F. Our results suggest that IHF exerts this effect by positively effecting transcription of the transfer (tra) operon of the plasmid.
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Achtman M, Manning PA, Kuseck B, Schwuchow S, Willetts N (1980) A genetic analysis of F sex factor cistrons needed for surface exclusion in E. coli. J Mol Biol 138:779–795
Beutin L Manning PA, Achtman M, Willetts N (1981) sfrA and sfrB products of E. coli K12 are transcriptional control factors. J Bacteriol 145:840–844
Bushman W, Thompson J, Vargas L, Landy A (1986) Control of directionality in lambda site-specific recombination. Science 230:906–911
Caro L, Schnös M (1966) The attachment of the male specific bacteriophage f1 to sensitive strains of E. coli. Proc Natl Acad Sci USA 56:126–132
Craig N, Nash HA (1984) E. coli integration host factor bind to specific sites in DNA. Cell 39:707–716
Flamm EL, Weisberg RA (1985) Primary structure of the hip gene of E. coli and of its products, the beta subunit of integration host factor. J Mol Biol 183:117–128
Friden P, Voelkel K, Sternglanz R, Freundlich M (1984) Reduced expression of the isoleucine and valine enzymes in integration host factor mutants of E. coli. J Mol Biol 172:573–579
Galas D, Chandler M (1982) Structure and stability of Tn9-mediated cointegrates. J Mol Biol 154:245–272
Gamas P, Galas D, Chandler M (1985) DNA sequence at the end of IS1 required for transposition. Nature 317:458–460
Gamas P, Burger A-C, Churchward G, Caro L, Galas D, Chandler M (1986) Replication of pSC101: effects of mutations in the E. coli DNA binding protein IHF. Mol Gen Genet 204:85–89
Guyer MS, Reed RR, Steitz JA, Low KB (1981) Identification of a sex factor affinity site in E. coli as gamma-delta. Cold Spring Harbor Symp Quant Biol 45:135–140
Heffron F, So M, McCarthy BJ (1978) In vitro mutagenesis of a circular DNA molecule by using synthetic restriction sites. Proc Natl Acad Sci USA 75:6012–6016
Helmuth R, Achtman M (1975) Operon structure of DNA transfer cistrons on the F sex factor. Nature 257:652–656
Johnson D, Willetts N (1980) Construction and characterisation of multicopy plasmids containing the entire F transfer region. Plasmid 4:292–304
Krause HM, Higgins PN (1986) Positive and negative regulation of the Mu operator by Mu repressor and E. coli integration host factor. J Biol Chem 261:3744–3752
McEwen J, Silverman P (1980) Chromosomal mutations of E. coli that alter expression of conjugal plasmid functions. Proc Natl Acad Sci USA 77:513–517
Mahajna J, Oppenheim AB, Rattray A, Gottesman M (1986) Translation initiation of bacteriophage lambda gene cII requires integration host factor. J Bacteriol 165:167–174
Mechulam Y, Fayat G, Blanquet S (1985) Sequence of the E. coli pheST operon and identification of the himA gene. J Bacteriol 163:787–791
Miller HI (1985) Primary structure of the himA gene of E. coli: homology with DNA binding protein Hu and association with the phenyalanine-tRNA synthetase operon. Cold Spring Harbor Symp Quant Biol 49:691–698
Prentki P, Gamas P, Galas DJ, Chandler M (1987) Functions of the end of JS1. In: Kelly (ed) Replication and Recombination and transposition. UCLA Symposium on Molecular and Cellular Biology, Benjamin Inc (in press)
Willetts N, Skurray R (1980) The conjugation system of F-like plasmids. Annu Rev Genet 14:41–76
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Communicated by R. Devoret
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Gamas, P., Caro, L., Galas, D. et al. Expression of F transfer functions depends on the Escherichia coli integration host factor. Mol Gen Genet 207, 302–305 (1987). https://doi.org/10.1007/BF00331593
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DOI: https://doi.org/10.1007/BF00331593