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Separation of chlorophyll a and metalloporphyrins by HPLC in normal-phase mode using a diol-phase

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Abstract

The isolation of high-purity chlorophyll a was achieved by HPLC using a micro-preparative column with a diol-phase (Polyol, Si 100, 5 μm) in normal-phase mode. The procedure included the extraction of photosynthetic pigments from the leaves of stinging nettles and their partial purification as dioxane-water-chlorophyll-aggregates prior to the isolation by micro-preparative HPLC. Freeze-drying resulted in a flaky chlorophyll a, which was free of water. Each step of isolation was controlled by using an analytical HPLC-column with a diol-phase and a photodiode array detector. Synthesized metalloporphyrins (metal(II)-ion = Mg2+, Zn2+, Cu2+, Ni2+) with structures analogous to chlorophyll were also isolated by means of the micro-preparative HPLC-column containing a diol-phase. The purity of the obtained porphyrins was monitored with an analytical HPLC-column.

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References

  1. Svec WA (1978) The isolation, preparation, characterization, and estimation of the chlorophylls and the bacteriochlorophylls. In: Dolphin D (ed) The porphyrins, vol V, part C. Academic Press, New York, pp 341–399

    Google Scholar 

  2. Scholz B, Ballschmiter K (1981) J Chromatogr 208:148–155

    Google Scholar 

  3. Köble R (1991) Dr. rer. nat. Thesis, University of Ulm

  4. Macherey & Nagel (1991) Catalog HPLC Flüssigkeitschromatographie, Düren, pp 17–20

  5. Iriyama K, Ogura N, Takamiya A (1974) J Biochem 76:901–904

    Google Scholar 

  6. Iriyama K, Shiraki M (1977) Chem Lett 787–788

  7. Iriyama K, Shiraki M (1979) J Liquid Chromatogr 2:255–276

    Google Scholar 

  8. Katz JJ, Ballschmiter K (1968) Angew Chem 80:283–284

    Google Scholar 

  9. Brockmann H Jr (1978) Stereochemistry and absolute configuration of chlorophylls and linear tetrapyrroles. In: Dolphin D (ed) The porphyrins, vol II, part B. Academic Press, New York, pp 287–326

    Google Scholar 

  10. Wasielewski MR, Svec WA (1980) J Org Chem 45:1969–1974

    Google Scholar 

  11. Falk JE (1964) Porphyrins and metalloporphyrins. Elsevier, Amsterdam

    Google Scholar 

  12. Hynninen PH, Sievers G (1981) Z Naturforsch 36:1000–1009

    Google Scholar 

  13. Ballschmiter K, Katz JJ (1969) J Am Chem Soc 91:2661–2677

    Google Scholar 

  14. Chow H-C, Serlin R, Strouse CE (1975) J Am Chem Soc 97:7230–7242

    Google Scholar 

  15. Strouse CE (1976) Proc Inorg Chem 21:159–177

    Google Scholar 

  16. Cotton FA, Wilkinson G (1980) Advanced inorganic chemistry, 4th edn. Wiley, New York

    Google Scholar 

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Authors and Affiliations

  1. Abteilung Analytische Chemie und Umweltchemie, Universität Ulm, Albert-Einstein-Allee 11, D-89069, Ulm, Germany

    Dieter Drexler & Karlheinz Ballschmiter

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  1. Dieter Drexler
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  2. Karlheinz Ballschmiter
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Drexler, D., Ballschmiter, K. Separation of chlorophyll a and metalloporphyrins by HPLC in normal-phase mode using a diol-phase. Fresenius J Anal Chem 348, 590–594 (1994). https://doi.org/10.1007/BF00323938

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  • DOI: https://doi.org/10.1007/BF00323938

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