Abstract
Formation and accumulation of lipolysosomes in developing chick hepatocytes were investigated by means of electron microscopy in combination with biochemical analyses of the lipid composition in liver homogenates. The lipolysosomes occurred with highest frequency from days 11 to 14 of incubation. They were usually small and electron-dense, but during development they gradually enlarged with an accompanying reduction in electron density. Coinciding with this enlargement was an accumulation of esterified cholesterol in the liver homogenates. After hatching, an immediate decrease in the size and number of lipolysosomes occurred along with a reduction in the concentration of esterified cholesterol, of which only a very small amount remained by 9 days of age. Instead of cholesterol, triglycerides subsequently increased in concentration and accounted for the major lipid content of the liver homogenates. In keeping with the ultrastructural changes, the total volume of cytoplasmic lipid droplets rapidly increased with increasing age. This transient accumulation of esterified cholesterol within lipolysosomes may be attributed to an excessive uptake and processing of plasma lipoprotein particles, probably derived from the egg yolk. This concept is supported by an abundance of coated pits, endosomes and multivesicular bodies in the embryonic hepatocytes.
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Assman G, Fredrickson DS (1983) Acid lipase deficiency: Wolman's disease and cholesterylester storage disease. In: Stanbury JB, Wyngaarden JB, Fredrickson DS, Goldstein JL, Brown MS (eds) The metabolic basis of inherited disease. McGraw-Hill, New York, pp 803–819
Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917
Chao Y-S, Jones AL, Hradek GT, Windler EET, Havel RJ (1981) Autoradiographic localization of the sites of uptake, cellular transport, and catabolism of low density lipoproteins in the liver of normal and estrogen-treated rats. Proc Natl Acad Sci USA 78:597–601
Drevon CA, Hovig T (1977) The effects of cholesterol/fat feeding on lipid levels and morphological structures in liver, kidney and spleen in guinea pigs. Acta Pathol Microbiol Scand Sect A 85:1–18
Duncombe WG (1964) The colorimetric micro-determination of non-esterified fatty acids on plasma. Clin Chim Acta 9:122–125
Gomori G (1952) Microscopic histochemistry. The University of Chicago Press, Chicago 3:193
Goodridge AG (1968) Conversion of U-14C glucose into carbon dioxide, glycogen, cholesterol and fatty acids in liver slices from embryonic and growing chicks. Biochem J 108:655–661
Havel RJ, Hamilton RL (1988) Hepatocytic lipoprotein receptors and intracellular lipoprotein catabolism. Hepatology 8:1689–1704
Hayashi H, Sternlieb I (1975) Lipolysosomes in human hepatocytes. Ultrastructural and cytochemical studies of patients with Wilson's disease. Lab Invest 33:1–7
Hayashi H, Winship DH, Sternlieb I (1977) Lipolysosomes in human liver: distribution in livers with fatty infiltration. Gastroenterology 73:651–654
Hayashi H, Sameshima Y, Lee M, Hotta Y, Kosaka T (1983) Lipolysosomes in human hepatocytes: their increase in number associated with serum level of cholesterol in chronic liver disease. Hepatology 3:221–225
Helenius A, Mellman I, Wall D, Hubbard A (1983) Endosomes. Trends Biochem Sci 8:245–250
Jones AL, Hradek GT, Hornick C, Renaud G, Windler EET, Havel RJ (1984) Uptake and processing of remnants of chylomicrons and very low density lipoproteins by rat liver. J Lipid Res 25:1151–1158
Kanai M (1989) Ultrastructural and biochemical studies of lipolysis by lipolysosomes in chick hepatocytes. Cell Tissue Res 255:559–565
Kanai M, Watari N, Sugawara E, Hasegawa N (1987) Ultrastructural study of the mechanism of lipid accumulation within lipolysosomes in chick embryonic hepatocytes. J Clin Electron Microscopy 20:548–549
Karrer HE, Cox J (1961) Electron microscope observations on chick embryo liver: glycogen, bile canaliculi, inclusion bodies and hematopoiesis. J Ultrastruct Mol Struct Res 5:116–141
Lake BD, Patrick AD (1970) Wolman's disease: deficiency of E 600-resistant acid esterase activity with storage of lipids in lysosomes. J Pediatr 76:262–266
Lee M, Hayashi H, Kato S, Sameshima Y, Hotta Y (1982) Egg yolk-induced lipolysosome proliferation and fat infiltration of rat liver. Lab Invest 47:194–197
Lough J, Fawcett J, Wiegensberg B (1970) Wolman's disease. An electron microscopic, histochemical and biochemical study. Arch Pathol 89:103–110
Luft JH (1961) Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol 9:409–414
Millonig G (1962) Further observations on a phosphate buffer for osmium solutions in fixation. In: Fifth International Congress of Electron Microscopy, vol. 2. Academic Press, Philadelphia, p 8
Moore JH, Doran BM (1962) Lipid metabolism in the normal and vitamin B12-deficient chick embryo. Biochem J 84:506–513
Narbaitz R, Kacew S, Burke B (1980) Ultrastructural and biochemical alterations in the livers from chick embryos maintained in shell-less culture. Anat Embryol (Berl) 159:307–316
Nehemiah JL, Novikoff AB (1974) Unusual lysosomes in hamster hepatocytes. Exp Mol Pathol 21:398–423
Nehemiah JL, Novikoff AB (1973) Unusual lysosomes in hamster hepatocytes. J Cell Biol 59:246a
Noble RC, Connor K, Smith WK (1984) The synthesis and accumulation of cholesteryl esters by the developing embryo of the domestic fowl. Poult Sci 63:558–564
Noble RC, Moore JH (1964) Studies on the lipid metabolism of the chick embryo. Can J Biochem 42:1728–1741
Novikoff AB (1964) GERL, its form and function in neurons of rat spinal ganglia. Biol Bull 127:358
Richmond M (1973) Preparation and properties of a cholesterol oxidase from Nocardia sp. and its application to the enzymatic assay of total cholesterol in serum. Clin Chem 19:1350–1356
Sandstrom B, Westman J (1971) Ultrastructure of the developing chicken liver before hatching. Z Zellforsch 117:516–525
Sato T (1968) A modified method for lead staining of thin sections. J Electron Microsc (Tokyo) 17:158–159
Schjeide OA (1963) Lipoprotein of the fowl-serum, egg yolk and intracellular. Prog Chem Fats Other Lipids 6:251–289
Stephens RJ, Bils RF (1967) Ultrastructural changes in the developing chick liver. 1. General cytology. J Ultrastruct Mol Struct Res 18:456–474
Takayama M, Itoh S, Nagasaki T, Tanimizu I (1977) A new enzymatic method for determination of serum choline-containing phospholipds. Clin Chim Acta 79:93–98
Watson ML (1958) Staining of tissue sections for electron microscopy with heavy metals. J Biophys Biochem Cytol 4:475–478
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Kanai, M., Watari, N., Soji, T. et al. Formation and accumulation of lipolysosomes in developing chick hepatocytes. Cell Tissue Res 275, 125–132 (1994). https://doi.org/10.1007/BF00305380
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DOI: https://doi.org/10.1007/BF00305380