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Characterization of a DMSP-degrading bacterial isolate from the Sargasso Sea

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Abstract

A bacterium which cleaves dimethylsulfoniopropionate (DMSP) to form dimethylsulfide (DMS) was isolated from surface Sargasso Sea water by a DMSP enrichment technique. The isolate, here designated LFR, is a Gram-negative, obligately aerobic, rod-shaped, carotenoid-containing bacterium with a DNA G+C content of 70%. Sequencing and comparison of its 16S ribosomal ribonucleic acid (rRNA) with that of known eubacteria revealed highest similarity (91% unrestricted sequence similarity) to Roseobacter denitrificans (formerly Erythrobacter species strain OCh114), an aerobic, bacteriochlorophyll-containing marine representative of the α-Proteobacteria. However, physiological differences between the two bacteria, and the current lack of other characterized close relatives, preclude assignment of strain LFR to the Roseobacter genus. Screening of fifteen characterized marine bacteria revealed only one, Pseudomonas doudoroffii, capable of degrading DMSP to DMS. Strain LFR is deposited with the American Type Culture Collection (ATCC 51258) and 16S rRNA sequence data are available under GenBank accession number 15345.

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Authors and Affiliations

  1. Chemistry Department, Woods Hole Oceanographic Institution, 02543, Woods Hole, MA, USA

    Kathleen M. Ledyard

  2. Department of Biology, University of California, Santa Barbara, 93106, Santa Barbara, CA, USA

    Edward F. DeLong

  3. Biology Department, Woods Hole Oceanographic Institution, 02543, Woods Hole, MA, USA

    John W. H. Dacey

Authors
  1. Kathleen M. Ledyard
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  2. Edward F. DeLong
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  3. John W. H. Dacey
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Contribution no. 8337 of the Woods Hole Oceanographic Institution

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Ledyard, K.M., DeLong, E.F. & Dacey, J.W.H. Characterization of a DMSP-degrading bacterial isolate from the Sargasso Sea. Arch. Microbiol. 160, 312–318 (1993). https://doi.org/10.1007/BF00292083

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  • DOI: https://doi.org/10.1007/BF00292083

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