Summary
A translocation mapping procedure was used to map the Glu-1 genes, controlling high-molecular-weight (HMW) glutenin subunits on the long arms of group 1 wheat chromosomes, with respect to their centromeres. The Glu-A1 genes on chromosome arm 1AL were linked with the centromere but gave very different map distances (in cM) of 10.2 ± 3.5 and 33.9 ± 5.4 with test-cross and F2 progeny, respectively. The centromere-Glu-B1 and centromere-Glu-D1 map distances were estimated to be 28.1 ± 2.8 and 30.9 ± 2.7, respectively, using test-cross seeds. A telocentric mapping procedure gave a reduced map distance of 22 ± 3.5 for the centromere-Glu-D1 interval. Possible reasons for the differences between the mapping results obtained with test-cross and F2 seeds and with the translocation and telocentric procedures are discussed.
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References
Bietz JA, Shepherd KW, Wall JS (1975) Single-kernel analysis of glutenin: use in wheat genetics and breeding. Cereal Chem 52:513–532
Branlard G, Le Blanc A (1985) Glutenins of bread and durum wheat cultivars in France. Agronomie 5:467–477
Brown JWS, Kemble RJ, Law CN, Flavell RB (1979) Control of endosperm proteins in Triticum aestivum (var Chinese Spring) and Aegilops umbellulata by homoeologous group I chromosomes. Genetics 93:189–200
Brown JWS, Law CN, Worland AJ, Flavell RB (1981) Genetic variation in wheat endosperm proteins: an analysis by two-dimensional electrophoresis using intervarietal chromosomal substitution lines. Theor Appl Genet 59:361–371
Driscoll CJ, Sears ER (1971) Individual addition of the chromosomes of ‘Imperial’ rye to wheat. Agron Abstr, p 6
Endrizzi JE, Kohel RJ (1966) Use of telosomes in mapping three chromosomes in cotton. Genetics 54:535–550
Galili G, Feldman M (1983a) Genetic control of endosperm proteins in wheat. I. The use of high resolution one-dimensional gel electrophoresis for the allocation of genes coding for endosperm protein subunits in the common wheat cultivar Chinese Spring. Theor Appl Genet 64:97–101
Galili G, Feldman M (1983b) Genetic control of endosperm proteins in wheat. II. Variation of HMW glutenin and gliadin subunits of Triticum aestivum. Theor Appl Genet 66:77–86
Haldane JBS (1919) The probable errors of calculating linkage values, and the most accurate method of determining gametic from certain zygotic series. J Genet 8:291–298
Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175
Lawrence GJ, Shepherd KW (1981a) Chromosomal location of genes controlling seed proteins in species related to wheat. Theor Appl Genet 59:25–31
Lawrence GJ, Shepherd KW (1981b) Inheritance of glutenin protein subunits of wheat. Theor Appl Genet 60:333–337
Mather K (1936) Types of linkage data and their value. Ann Eugen 7:251–264
Mather K (1951) The measurement of linkage in heredity. Methuen's Monographs on Biological Subjects, 2nd edn. London
Orth RA, Bushuk W (1974) Studies of glutenin. VI. Chromosomal location of genes coding for subunits of glutenin of common wheat. Cereal Chem 51:118–126
Payne PI, Lawrence GJ (1983) Catalogue of alleles for the complex gene loci, Glu-A1, Glu-B1 and Glu-D1 which code for high-molecular-weight subunits of glutenin in hexaploid wheat. Cereal Res Commun 11:29–35
Payne PI, Law CN, Mudd EE (1980) Control by homoeologous group 1 chromosomes of the high-molecular weight subunits of glutenin, a major protein of wheat endosperm. Theor Appl Genet 58:113–120
Payne PI, Holt LM, Law CN (1981) Structural and genetical studies on the high-molecular weight subunits of wheat glutenin. 1. Allelic variation in subunits amongst varieties of wheat (Triticum aestivum). Theor Appl Genet 60:229–236
Payne PI, Holt LM, Worland AJ, Law CN (1982) Structural and genetical studies on the high-molecular-weight sub-units of wheat glutenin. 3. Telocentric mapping of the subunit genes on the long arms of homoeologous group 1 chromosomes. Theor Appl Genet 63:129–138
Payne PI, Holt LM, Jackson EA (1984) Genetical analysis of wheat endosperm storage proteins. In: Graveland A, Moonen JHE (eds) Proc 2nd Int Workshop Gluten Proteins. Wageningen, The Netherlands, pp 111–119
Sears ER (1954) The aneuploids of common wheat. Mo Agric Exp Stn Res Bull 572:1–58
Sears ER (1962) The use of telocentrics in linkage mapping. Genetics 47:983
Sears ER (1966) Chromosome mapping with the aid of telocentrics. In: Proc 2nd Int Wheat Genet Symp. Lund, Sweden. Hereditas (Suppl) 2:370–381
Sears ER (1972) Reduced proximal crossing-over in telocentric chromosomes of wheat. Genet Iber 24:233–239
Sears ER, Sears LMS (1978) The telocentric chromosomes of common wheat. In: Ramanujam S (ed) Proc 5th Int Wheat Genet Symp. Indian Soc Genet Plant Breed, New Delhi, pp 389–407
Sears ER, Loegering WQ, Rodenhiser HA (1957) Identification of chromosomes carrying genes for stem rust resistance in four varieties of wheat. Agron J 49:208–212
Shepherd KW (1973) Homoeology of wheat and alien chromosomes controlling endosperm protein phenotypes. In: Sears ER, Sears LMS (eds) Proc 4th Int Wheat Genet Symp. University of Missouri, Columbia, pp 745–760
Singh NK, Shepherd KW (1984) Mapping of the genes controlling high-molecular-weight glutelin subunits of rye on the long arm of chromosome 1R. Genet Res 44:117–123
Singh NK, Shepherd KW (1985) The structure and genetic control of a new class of disulphide-linked proteins in wheat endosperm. Theor Appl Genet 71:79–92
Singh NK, Shepherd KW (1988) Linkage mapping of genes controlling endosperm storage proteins in wheat. 1. Genes on the short arms of group 1 chromosomes. Theor Appl Genet 75:628–641
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Communicated by R. Riley
Portion of the Ph.D. thesis submitted by the senior author
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Singh, N.K., Shepherd, K.W. Linkage mapping of genes controlling endosperm storage proteins in wheat. Theoret. Appl. Genetics 75, 642–650 (1988). https://doi.org/10.1007/BF00289133
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DOI: https://doi.org/10.1007/BF00289133