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Genetic interactions among genes involved in the STT4-PKC1 pathway of Saccharomyces cerevisiae

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Abstract

Loss of yeast protein kinase C function results in three distinct phenotypes: staurosporine sensitivity, cell lysis and blockage of cell cycle progression at the G2/M boundary. Genetic analysis of the PKC1/STT1 protein kinase C gene and its interactions with STT4, encoding an upstream phosphatidylinositol 4-kinase, and BCK1, encoding a downstream protein kinase, reveal that they form part of a single pathway. However, the BCK1-20 mutation (a gain-of-function mutation of BCK1) or overexpression of PKC1 cannot suppress all of the phenotypes caused by the loss of STT4 function, strongly suggesting the existence of a branch point between STT4 and PKC1. We also describe the MSS4 gene, a multicopy suppressor of the temperature-sensitive stt4-1 mutation. MSS4 is predicted to encode a hydrophilic protein of 779 amino acid residues and is essential for cell growth. Based on genetic and biochemical data, we suggest that MSS4 acts downstream of STT4, but in a pathway that does not involve PKC1.

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Authors and Affiliations

  1. Department of Biology, Faculty of Science, University of Tokyo, Hongo, Bunkyo-ku, 113, Tokyo, Japan

    Satoshi Yoshida, Yoshikazu Ohya, Akihiko Nakano & Yasuhiro Anraku

  2. Department of Genetics, Stanford University School of Medicine, 94305-5120, Stanford, CA, USA

    Yoshikazu Ohya

Authors
  1. Satoshi Yoshida
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  2. Yoshikazu Ohya
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  3. Akihiko Nakano
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  4. Yasuhiro Anraku
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Additional information

Communicated by C.P. Hollenberg

GenBank accession number: The accession number for the MSS4 sequence reported in this paper is D13716.

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Yoshida, S., Ohya, Y., Nakano, A. et al. Genetic interactions among genes involved in the STT4-PKC1 pathway of Saccharomyces cerevisiae . Molec. Gen. Genet. 242, 631–640 (1994). https://doi.org/10.1007/BF00283416

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  • DOI: https://doi.org/10.1007/BF00283416

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