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Two new species of anaerobic oxalate-fermenting bacteria, Oxalobacter vibrioformis sp. nov. and Clostridium oxalicum sp. nov., from sediment samples

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Abstract

Two types of new anaerobic bacteria were isolated from anoxic freshwater sediments. They grew in mineral medium with oxalate as sole energy source and with acetate as main carbon source. Oxalate as well as oxamate (after deamination) were decarboxylated to formate with growth yields of 1.2–1.4 g dry cell matter per mol oxalate degraded. No other organic or inorganic substrates were used, and no electron acceptors were reduced. Strain WoOx3 was a Gramnegative, non-sporeforming, motile vibrioid rod with a guanine-plus-cytosine content of the DNA of 51.6 mol%. It resembled the previously described genus Oxalobacter, and is described as a new species, O. vibrioformis. Strain AltOx1 was a Gram-positive, spore-forming, motile rod with a DNA base ratio of 36.3 mol% guanine-plus-cytosine. This isolate is described as a new species of the genus Clostridium, C. oxalicum.

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References

  • AllisonMJ, LittedikeET, JamesLF (1977) Changes in ruminal oxalate degradation rates associated with adaptation to oxalate ingestion. J Anim Sci 45: 1173–1179

    Google Scholar 

  • AllisonMJ, DawsonKA, MayberryWR, FossJG (1985) Oxalobacter formigenes gen. nov., sp. nov.: oxalate-degrading anaerobes that inhabit the gastrointestinal tract. Arch Microbiol 141: 1–7

    Google Scholar 

  • AnantharamV, AllisonMJ, MaloneyPC (1989) Oxalate: formate exchange: The basis for energy coupling in Oxalobacter. J Biol Chem 264: 7244–7250

    Google Scholar 

  • BhatJV (1966) Enrichment culture technique. J Sci Ind Res New Delhi 25: 450–454

    Google Scholar 

  • BlendenDC, GoldbergHS (1965) Silver impregnation stain for Leptospira and flagella. J Bacteriol 89: 899–900

    Google Scholar 

  • ChandraTS, ShethnaYI (1975) Isolation and characterization of some new oxalate-decomposing bacteria. Antonie van Leeuwenhoek J Microbiol Serol 41: 465–477

    Google Scholar 

  • ClineJD (1969) Spectrophotometric determination of hydrogen sulfide in natural waters. Limnol Oceanogr 14: 454–458

    Google Scholar 

  • DawsonKA, AllisonMJ, HartmanPA (1980) Isolation and some characteristics of anaerobic oxalate-degrading bacteria from the rumen. Appl Environ Microbiol 40: 833–839

    Google Scholar 

  • DehningI, SchinkB (1989) Malonomonas rubra gen. nov. sp. nov., a microaerotolerant anaerobic bacterium growing by decarboxylation of malonate. Arch Microbiol 151: 427–433

    Google Scholar 

  • DehningI, StiebM, SchinkB (1989) Sporomusa malonica sp. nov., a homoacetogenic bacterium growing by decarboxylation of malonate and succinate. Arch Microbiol 151: 421–426

    Google Scholar 

  • DeLeyJ (1970) Reexamination of the association between melting point, buoyant density and the chemical base composition of deoxyribonucleic acid. J Bacteriol 101: 738–754

    Google Scholar 

  • DiekertG, ThauerRK (1978) Carbon monoxide oxidation by Clostridium thermoaceticum and C. formicoaceticum. J Bacteriol 136: 597–606

    Google Scholar 

  • DiekertG, SchraderE, HarderW (1986) Energetics of CO formation and CO oxidation in cell suspensions of Acetobacterium woodii. Arch Microbiol 144: 386–392

    Google Scholar 

  • DijkhuizenL, WiersmaM, HarderW (1977) Energy production and growth of Pseudomonas oxalaticus OX1 on oxalate and formate. Arch Microbiol 115: 229–236

    Google Scholar 

  • HilpertW, SchinkB, DimrothP (1984) Life by a new decarboxylation-dependent energy conservation mechanism with Na+ as coupling ion. EMBO J 3: 1665–1670

    Google Scholar 

  • HodgkinsonA (1977) Oxalic acid in biology and medicine. Academic Press, Inc, New York

    Google Scholar 

  • JakobyWB, BhatJV (1958) Microbial metabolism of oxalic acid. Bacteriol Rev 22: 75–80

    Google Scholar 

  • KrzyckiJA, ZeikusJG (1984) Characterization and purification of carbon monoxide dehydrogenase from Methanosarcina barkeri. J Bacteriol 158: 231–237

    Google Scholar 

  • LangE, LangH (1972) Spezifische Farbreaktion zum direkten Nachweis der Ameisensäure. Z Anal Chem 260: 8–10

    Google Scholar 

  • MageeCM, RodeheaverG, EdgertonMT, EdlichRF (1975) A more reliable Gram staining technic for diagnosis of surgical infections. Am J Surg 130: 341–346

    Google Scholar 

  • MarmurJ (1961) A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3: 208–218

    Google Scholar 

  • MorrisMP, Garcia-RiveraJ (1955) The destruction of oxalate by rumen contents of cows. J Dairy Sci 38: 1169

    Google Scholar 

  • PfennigN, TrüperHG (1981) Isolation of members of the families Chromatiaceae and Chlorobiaceae. In: StarrMP, StolpH, TrüperHG, BalowsA, SchlegelHG (eds) The prokaryotes, vol I. Springer, Berlin Heidelberg New York, pp 279–289

    Google Scholar 

  • PostgateJR (1963) A strain of Desulfovibrio able to use oxamate. Arch Mikrobiol 46: 287–295

    Google Scholar 

  • QuayleJR (1961) Metabolism of C1 compounds in autotrophic and heterotrophic microorganisms. Ann Rev Microbiol 15: 119–152

    Google Scholar 

  • SchinkB, PfennigN (1982) Propionigenium modestum gen. nov. sp. nov., a new strictly anaerobic, nonsporing bacterium growing on succinate. Arch Microbiol 133: 209–216

    Google Scholar 

  • SmithRL, OremlandRS (1983) Anaerobic oxalate degradation: widespread natural occurrence in aquatic sediments. Appl Environ Microbiol 46: 106–113

    Google Scholar 

  • SmithRL, StrohmaierFE, OremlandRS (1985) Isolation of anaerobic oxalate-degrading bacteria from freshwater lake sediments. Arch Microbiol 141: 8–13

    Google Scholar 

  • ThauerRK, JungermannK, DeckerK (1977) Energy conservation of chemotrophic anaerobic bacteria. Bacteriol Rev 41: 100–180

    Google Scholar 

  • ThimannKV, BonnerWD (1950) Organic acid metabolism. Ann Rev Plant Physiol 1: 75–108

    Google Scholar 

  • UffenRL (1976) Anaerobic growth of a Rhodopseudomonas species in the dark with carbon monoxide as sole carbon and energy substrate. Proc Natl Acad Sci USA 73: 3298–3302

    Google Scholar 

  • WiddelF, PfennigN (1981) Studies on dissimilatory sulfate-reducing bacteria that decompose fatty acids. I. Isolation of a new sulfate-reducer enriched with acetate from saline environments. Description of Desulfobacter postgatei gen. nov. sp. nov. Arch Microbiol 129: 395–400

    Google Scholar 

  • WiddelF, KohringGW, MayerF (1983) Studies on dissimilatory sulfate-reducing bacteria that decompose fatty acids. III. Characterization of the filamentous gliding Desulfonema limicola gen. nov. sp. nov., and Desulfonema magnum sp. nov. Arch Microbiol 134: 286–294

    Google Scholar 

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Dehning, I., Schink, B. Two new species of anaerobic oxalate-fermenting bacteria, Oxalobacter vibrioformis sp. nov. and Clostridium oxalicum sp. nov., from sediment samples. Arch. Microbiol. 153, 79–84 (1989). https://doi.org/10.1007/BF00277545

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  • DOI: https://doi.org/10.1007/BF00277545

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