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The construction in vitro of transducing derivatives of phage lambda

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Summary

Methods are described for the construction of plaque-forming, transducing derivatives of phage lambda, using appropriate receptor genomes and fragments of DNA generated by the restriction enzymes endo R.EcoRI and endo R.HindIII. The general properties of the transducing derivatives are described and discussed. Plaque-forming phages carrying the E. coli trp, his, cysB, thyA, supD, supE, supF, hsd, tna and lig genes have been isolated.

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Authors and Affiliations

  1. Department of Molecular Biology, University of Edinburgh, King's Buildings, Mayfield Road, EH9 3JR, Edinburgh

    Kathleen Borck, Jean D. Beggs, W. J. Brammar, A. S. Hopkins & Noreen E. Murray

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  1. Kathleen Borck
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  2. Jean D. Beggs
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  3. W. J. Brammar
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  4. A. S. Hopkins
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  5. Noreen E. Murray
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Communicated by B.A. Bridges

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Borck, K., Beggs, J.D., Brammar, W.J. et al. The construction in vitro of transducing derivatives of phage lambda. Molec. Gen. Genet. 146, 199–207 (1976). https://doi.org/10.1007/BF00268089

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