Skip to main content
SpringerLink
Log in

Recombination between the extranuclear genes conferring oligomycin resistance and cold sensitivity in Aspergillus nidulans

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

Recombination has been demonstrated between the extranuclear loci (oliA1) and (cs67) of Aspergillus nidulans. The stability of the double mutant recombinant and the fact that it formed smaller colonies than either parent at the non-permissive temperature are strong evidence that physical recombination of the extranuclear DNA has occurred rather than simple mixing. A method has been developed for quantifying the extranuclear recombination frequency, thus providing a means of mapping the A. nidulans mitochondrial genome. The data obtained suggests that the two loci are not closely linked.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bertrand, H., Pittenger, T.H.: Isolation and classification of extranuclear mutants of Neurospora crassa. Genetics 71, 521–533 (1972a)

    Google Scholar 

  • Bertrand, H., Pittenger, T.H.: Complementation among cytoplasmic mutants of Neurospora crassa. Molec. gen. Genet. 117, 82–90 (1972b)

    Google Scholar 

  • Coen, D., Deutsch, J., Netter, P., Petrochilo, E., Slonimski, P. P.: Mitochondrial genetics I—Methodology and phenomenology. In: Control of organelle development. Symp. Soc. exp. Biol. 24, 449–496 (1970)

    Google Scholar 

  • Grivell, L.A., Netter, P., Borst, P., Slonimski, P.P.: Mitochondrial antibiotic resistance in yeast: ribosomal mutants resistant to chloramphenicol, erythromycin and spiramycin. Biochim. biophys. Acta (Amst.) 312, 358–367 (1973)

    Google Scholar 

  • Jagow, G. von, Weiss, H., Klingenberg, M.: Comparison of the respiratory chain of Neurospora crassa wild type and the mi-mutants mi-1 and mi-3. Eur. J. Biochem. 33, 140–157 (1973)

    Google Scholar 

  • Jinks, J.L.: Cytoplasmic inheritance in fungi. In: Methodology in basic genetics (Ed. Burdette, W.J.), p. 325–354. San Francisco: Holden-Day Inc. 1963

    Google Scholar 

  • Mackintosh, M.E., Pritchard, R.H.: The production and replica-plating of micro-colonies of Aspergillus nidulans. Genet. Res. 4, 320–322 (1963)

    Google Scholar 

  • Mets, L., Bogorad, L.: Altered chloroplast ribosomal proteins associated with erythromycin-resistant mutations in two genetic systems of Chlamydomonas reinhardi. Proc. nat. Acad. Sci. (Wash.) 69, 3779–3783 (1972)

    Google Scholar 

  • Michaelis, G., Petrochilo, E., Slonimski, P.P.: Mitochondrial genetics III. Recombined molecules of mitochondrial DNA obtained from crosses between cytoplasmic petite mutants of Saccharomyces cerevisiae: physical and genetic characterisation. Molec. gen. Genet. 123, 51–65 (1973)

    Google Scholar 

  • Mitchell, C.H., Bunn, C.L., Lukins, H.B., Linnane, A.W.: Biogenesis of mitochondria 23. The biochemical and genetic characteristcs of two different oligomycin resistant mutants of Saccharomyces cerevisiae under the influence of cytoplasmic genetic modification. J. Bioenergetics 4, 161–177 (1973)

    Google Scholar 

  • Rank, G.H., Bech-Hansen, N.T.: Somatic segregation, recombination, asymmetrical distribution and complementation tests of cytoplasmically-inherited antibiotic-resistance mitochondrial markers in S. cerevisiae. Genetics 72, 1–15 (1972)

    Google Scholar 

  • Rowlands, R.T., Turner, G.: Nuclear and extranuclear inheritance of oligomycin resistance in Aspergillus nidulans. J. gen. Microbiol. 75, XIX (1973a)

    Google Scholar 

  • Rowlands, R.T., Turner, G.: Nuclear and extranuclear inheritance of oligomycin resistance in Aspergillus nidulans. Molec. gen. Genet. 126, 201–216 (1973b)

    Google Scholar 

  • Rowlands, R.T., Turner, G.: Interaction and recombination between extranuclear genetic elements of Aspergillus nidulans. Biochem. Soc. Trans. 2, 230–232 (1974a)

    Google Scholar 

  • Rowlands, R.T., Turner, G.: Physiological and biochemical studies of nuclear and extranuclear oligomycin-resistant mutants of Aspergillus nidulans. Molec. gen. Genet. 132, 73–88 (1974b)

    Google Scholar 

  • Schlanger, G., Sager, R., Ramanis, Z.: Mutation of a cytoplasmic gene in Chlamydomonas alters chloroplast ribosome function. Proc. nat. Acad. Sci. (Wash.) 69, 3551–3555 (1972)

    Google Scholar 

  • Shannon, C., Enns, R., Wheelis, L., Burchiel, K., Criddle, R.S.: Alterations in mitochondrial adenosine triphosphatase activity resulting from mutation of mitochondrial deoxyribonucleic acid. J. biol. Chem. 248, 3004–3011 (1973)

    Google Scholar 

  • Tait, A.: Altered mitochondrial ribosomes in an erythromycin resistant mutant of Paramecium. FEBS Letters 24, 117–120 (1972)

    Google Scholar 

  • Thomas, D.Y., Wilkie, D.: Recombination of mitochondrial drug-resistance factors in Saccharomyces cerevisiae. Biochem. biophys. Res. Commun. 30, 368–372 (1968)

    Google Scholar 

  • Waldron, C.: Cold-sensitive mutants of Aspergillus nidulans. Heredity 31, 135 (1973)

    Google Scholar 

  • Waldron, C., Roberts, C.F.: Cytoplasmic inheritance of a cold-sensitive mutant in Aspergillus nidulans. J. gen. Microbiol. 78, 379–381 (1973)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Bacteriology, The Medical School, University of Bristol, Bristol, England

    Robert T. Rowlands & Geoffrey Turner

Authors
  1. Robert T. Rowlands
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Geoffrey Turner
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by F. Kaudewitz

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rowlands, R.T., Turner, G. Recombination between the extranuclear genes conferring oligomycin resistance and cold sensitivity in Aspergillus nidulans . Molec. Gen. Genet. 133, 151–161 (1974). https://doi.org/10.1007/BF00264836

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00264836

Keywords

Search

Navigation