Summary
The many reports of phenotypic variation among plants regenerated from tissue culture suggest underlying alterations at the DNA level. This hypothesis was tested with protoplast-derived Solanum tuberosum plants. Random potato-DNA clones were used to probe the genome of individual plants at specific sites. Two out of twelve plants were shown to be variant by Southern-hybridisation with one of the tester-clones. As this clone turned out to represent 25S-rDNA, both somaclonal variants can be regarded as mutants deficient in ribosomal RNA-genes.
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Communicated by G. Wenzel
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Landsmann, J., Uhrig, H. Somaclonal variation in Solanum tuberosum detected at the molecular level. Theoret. Appl. Genetics 71, 500–505 (1985). https://doi.org/10.1007/BF00251196
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DOI: https://doi.org/10.1007/BF00251196