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Formylmethanofuran: tetrahydromethanopterin formyltransferase and N 5,N 10-methylenetetrahydromethanopterin dehydrogenase from the sulfate-reducing Archaeoglobus fulgidus: similarities with the enzymes from methanogenic Archaea

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Abstract

The sulfate-reducing Archaeoglobus fulgidus contains a number of enzymes previously thought to be unique for methanogenic Archaea. The purification and properties of two of these enzymes, of formylmethanofuran: tetrahydromethanopterin formyltransferase and of N 5,N 10-methylenetetrahydromethanopterin dehydrogenase (coenzyme F420 dependent) are described here. A comparison of the N-terminal amino acid sequences and of other molecular properties with those of the respective enzymes from three methanogenic Archaea revealed a high degree of similarity.

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Abbreviations

H4MPT:

tetrahydromethanopterin

F420 :

coenzyme

F420 :

formyltransferase, formylmethanofuran: tetrahydromethanopterin formyltransferase

methylene-H4MPT dehydrogenase:

N 5,N 10-methylenetetrahydromethanopterin dehydrogenase

methylene-H4MPT recductase:

N 5,N 10-methylenetetrahydromethanopterin reductase

cyclohydrolase:

N 5,N 10-methenyltetrahydromethanopterin cyclohydrolase

APS:

adenosine 5′-phosphosulfate

MOPS:

3-(N-morpholino) propane sulfonic acid

TRICINE:

N-tris(hydroxymethyl)methylglycine

MES:

morpholinoethanesulfonic acid

1 U:

1 μmol/min

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Authors and Affiliations

  1. Laboratorium für Mikrobiologie des Fachbereichs Biologie der Philipps-Universität Marburg, Karl-von-Frisch-Strasse, W-3550, Marburg/Lahn, Germany

    B. Schwörer, J. Breitung, A. R. Klein & R. K. Thauer

  2. Max-Planck-Institut für Terrestrische Mikrobiologie Marburg, Karl-von-Frisch-Strasse, W-3550, Marburg/Lahn, Germany

    B. Schwörer, J. Breitung, A. R. Klein & R. K. Thauer

  3. Lehrstuhl für Mikrobiologie, Universität Regensburg, Universitätsstrasse 31, W-8400, Regensburg, Germany

    K. O. Stetter

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  1. B. Schwörer
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  2. J. Breitung
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  4. K. O. Stetter
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  5. R. K. Thauer
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Schwörer, B., Breitung, J., Klein, A.R. et al. Formylmethanofuran: tetrahydromethanopterin formyltransferase and N 5,N 10-methylenetetrahydromethanopterin dehydrogenase from the sulfate-reducing Archaeoglobus fulgidus: similarities with the enzymes from methanogenic Archaea. Arch. Microbiol. 159, 225–232 (1993). https://doi.org/10.1007/BF00248476

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