Abstract
We studied the genome of an antarctic ice fish, Chionodraco hamatus, in order to detect highly repetitive DNAs that may play a role in heterochromatinization processes and sex chromosome differentiation. We used two different experimental approaches. Hybridization of a Bkm probe to genomic DNA showed slight differences between the two sexes. Using restriction enzymes, a Bgl II satellite (pIF) was isolated. In situ hybridization revealed a preferential localization of pIF on the centromeres and the telomeres of most chromosomes, as well as an interstitial band on the long arms of the neo-Y sex chromosome, where probably the hypothetical fusion took place. Dot-blot experiments showed that pIF is still present in species belonging to different families of the same suborder. Though preliminary, our results suggest a conservative nature of this DNA which might have played a definite functional role in the genome of these polar fishes.
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References
Arnemann J, Schafer R, Epplen JT (1986) Clustered GATA repeats (Bkm sequences) on the human Y chromosome. Hum Genet 73:301–303
Eastman JT, De Vries AL (1986) Antarctic fishes. Sci Am 254:106–114
Galetti PM, Foresti F (1986) Evolution of the ZZ/ZW system in Leporinus (Pisces, Anastomidae): role of constitutive heterochromatin. Cytogen Cell Gen 43:43–46
Griffith RG, Holland PWH (1990) A novel avian W-chromosome DNA repeat in the lesser black-backed gull (Larus fuscus). Chromosoma 99:243–250
Haaf T, Schmid M (1984) An early stage of ZW/ZZ sex chromosome differentiation in Poecilia sphenops melanistica (Poeciliidae, Cyprinodontiformes). Chromosoma 89:37–41
John B, Miklos GLG (1987) The eukaryote genome in development and evolution. London, Allen & Unwin
Jones KW (1984) The evolution of sex chromosomes and their consequences for the evolutionary process. Chromosomes Today 8:241–255
Levinson G, Marsh JL, Epplen JT, Gutman GA (1985) Cross-hybridizing snake satellite, Drosophila, and mouse DNA sequences may have arisen independently. Mol Biol Evol 2:494–504
Lonnberg E (1905) The fishes of the Swedish South Polar Expedition. Wiss Ergebn Schwed Südpolar-Exped 5:1–69
Maresca B, Patriarca E, Goldenberg C, Sacco M (1988) Heat shock and antarctic fishes: a molecular approach. Comp Biochem Physiol 90B:623–629
Mayr B, Schneizer D, Geber G (1984) NOR activity, heterochromatin differentiation and the Robertsonian polymorphisms in Sus scrofa L. J Hered 75:79–80
Miklos GLG, Matthaei KL, Reed KC (1989) Occurrence of the (GATA)n sequences in vertebrate and invertebrate genomes. Chromosoma 98:194–200
Morescalchi A (1992) Chromosomes, sex determination and environment in teleosteans. In: Dallai R (ed) Selected Symposia and Monographs UZI 6, Mucchi, Modena, pp 137–149
Morescalchi A, Pestarino M, Pisano E, Stanyon R, Stingo V, Morescalchi MA (1991) Preliminary data on the cytogenetics of Notothenioid fishes. In: Battaglia B, Bisol PM, Varotto M (eds), Proc First Meeting Biology in Antarctica, Rome 1989. Martello, E.U.P., Padova, pp 201–213
Morescalchi A, Hureau JC, Olmo E, Ozouf-Costaz C, Pisano E, Stanyon R (1992) A multiple sex chromosome system in Antarctic Ice Fishes. Polar Biol 11:655–661
Nanda I, Feichtinger W, Schmid MM, Schroder JH, Zischler H, Epplen JT (1990) Simple repetitive sequences are associated with differentiation of the sex chromosomes in the guppy fish. J Mol Evol 30:456–462
Nanda I, Schartl M, Feichtinger W, Epplen J, Schmid M (1992) Early stages of sex chromosome differentiation in fish analysed by single repetitive DNA sequences. Chromosoma 101:301–310
Ng R, Ness J, Carbon J (1986) Structural studies on centromeres in the yeast Saccharomyces cerevisiae. In: Wickner RB, Hiiebusch A, Lambowitz A, Gunzales IC, Hollander A (eds), Extrachromosomal elements in lower eukaryotes. Plenum Press, New York, pp 478–492
Pelliccia F, Di Castro M, Lanza V, Volpi EV, Rocchi A (1991) GATA repeats in the genome of Asellus aquaticus (Crustacea, Isopoda). Chromosoma 100:152–155
Radic ML, Lundgren K, Hamkalo B (1987) Curvature of mouse satellite DNA and condensation of heterochromatin. Cell 50:1101–1108
Schafer R, Boltz E, Becker A, Bartels F, Epplen JT (1986) The expression of the evolutionarily conserved GATA/GACA repeats in mouse tissues. Chromosoma 93:496–501
Singh L, Purdom IF, Jones KW (1980) Sex chromosomes associated satellite DNA: Evolution and conservation. Chromosoma 79:137–157
Singh L, Phillips C, Jones KW (1984) The conserved nucleotide sequences of Bkm, which define Sxr in the mouse, are transcribed. Cell 36:111–120
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
Tone M, Sakaki YY, Hashiguchi T, Mizuno S (1984) Genus specificity and extensive methylation of the W chromosome specific repetitive DNA sequences from the domestic fowl Gallus gallus domesticus. Chromosoma 89:228–237
Traut W (1987) Hypervariable Bkm DNA loci in a moth, Ephestia kuehniella: does transposition cause restriction fragment length polymorphism? Genetics 115:493–498
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Capriglione, T., Morescalchi, A., Olmo, E. et al. Satellite DNAs, Heterochromatin and sex chromosomes in Chionodraco hamatus (Channichthyidae, Perciformes). Polar Biol 14, 285–290 (1994). https://doi.org/10.1007/BF00239178
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DOI: https://doi.org/10.1007/BF00239178