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Regeneration of transgenic plants from the commercial apple cultivar Royal Gala

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Abstract

A transformation system was developed for the commercial apple (Malus X domestica Borkh.) cultivar Royal Gala. Leaf pieces from in vitro-grown shoots were cocultivated for 2 days with Agrobacterium tumefaciens strain LBA4404 containing the binary vectors pKIWI105 or pKIWI110. Shoots were produced on a shooting medium containing kanamycin (50 mg·L−1). A 2-day incubation period on kanamycin-free medium prior to antibiotic selection enhanced the regeneration of kanamycin-resistant shoots. The majority of the kanamycin-resistant shoots also expressed GUS (β-glucuronidase) activity. The putatively transformed shoots were rooted on a medium containing kanamycin (50 mg·L−1). Rooted plants were established in a greenhouse, and plants transformed with pKIWI110, which contains a mutant Arabidopsis acetolactate synthase gene, were shown to be resistant to the herbicide Glean™. Integration of T-DNA into the apple genome was confirmed by PCR and Southern hybridization analyses.

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Abbreviations

NAA:

α-naphthaleneacetic acid

IBA:

indole-3-butyric acid

BAP:

6-benzylaminopurine

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Authors and Affiliations

  1. Gene Transfer and Expression Group, The Horticulture and Food Research Institute of New Zealand Ltd., Mt Albert Research Centre, Private Bag 92169, Auckland, New Zealand

    Jia-Long Yao, Daniel Cohen, Ross Atkinson, Kim Richardson & Bret Morris

Authors
  1. Jia-Long Yao
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  2. Daniel Cohen
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  3. Ross Atkinson
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  4. Kim Richardson
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  5. Bret Morris
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Communicated by W. Parrott

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Yao, JL., Cohen, D., Atkinson, R. et al. Regeneration of transgenic plants from the commercial apple cultivar Royal Gala. Plant Cell Reports 14, 407–412 (1995). https://doi.org/10.1007/BF00234044

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  • DOI: https://doi.org/10.1007/BF00234044

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