Abstract
Plant regeneration from tissue cultures of summer squash, Cucurbita pepo L., cv. YC60, has been observed. Somatic embryos organized from shoot apex derived callus cultured on Murashige and Skoog (MS) medium supplemented with 1.2 mg/l 2,4,5-trichlorophenoxyacetic acid, 0.8 mg/l benzylaminopurine, and 0.1 mg/l kinetin. Embryos developed into plantlets by transfer of immature somatic embryos to MS medium with 0.05 mg/l NAA and 0.05 mg/l kinetin. Regenerated plants appeared morphologically normal and set fruits with seeds which could germinate normally.
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Abbreviations
- BAP:
-
6-benzylaminopurine
- 2,4-D:
-
2, 4dichlorophenoxyacetic acid
- IAA:
-
indole-3-acetic acid
- KN:
-
kinetin
- NAA:
-
β-naphthyleneacetic acid
- MS:
-
Murashige and Skoog
- 2,4,5-T:
-
2, 4,5-trichlorophenoxyacetic acid
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Chee, P.P. Somatic embryogenesis and plant regeneration of squash Cucurbita pepo L cv. YC 60. Plant Cell Reports 9, 620–622 (1991). https://doi.org/10.1007/BF00231801
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DOI: https://doi.org/10.1007/BF00231801