Summary
In the cat visual cortex, almost all parvalbumin-positive cells are GABAergic, and about 80% of the calbindin D-28K-positive neurons are also GABA-immunoreactive. About 37% of the GABAergic neurons contain parvalbumin and a smaller fraction (about 18%) contains calbindin. Furthermore, parvalbumin and calbindin are localized in two separate neuronal populations in the cat visual cortex, suggesting that two GABAergic populations can be distinguished, one containing parvalbumin and one containing calbindin. Double staining for parvalbumin and neuropeptides (CCK, SRIF and NPY), revealed no double-labeled cells, with the exception of a few SRIF- and parvalbumin-positive neurons. These results show that cortical GABAergic cells can be differentiated on basis of their calcium binding protein and neuropeptide immunoreactivity.
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References
Celio MR (1986) Parvalbumin in most γ-aminobutyric acid containing neurons of the rat cerebral cortex. Science 231:995–997
Demeulemeester H, Vandesande F, Orban GA, Brandon C, Vanderhaeghen JJ (1988) Heterogeneity of GABAergic cells in cat visual cortex. J Neurosci 8:988–1000
Demeulemeester H, Vandesande F, Orban GA, Heizmann CW, Pochet R (1989) Calbindin D-28K and parvalbumin immunoreactivity is confined to two separate neuronal subpopulations in the cat visual cortex, whereras partial coexistence is shown in the dorsal lateral geniculate nucleus. Neurosci Lett 99:6–11
Dierickx K, Vandesande F (1979) Immunocytochemical localization of somatostatin containing neurons in the rat hypothalamus. Cell Tissue Res 201:349–359
Hendrickson AE, Hunt SP, Wu J-Y (1981) Immunocytochemical localization of glutamic acid decarboxylase in monkey striate cortex. Nature 292:605–607
Hendry SHC, Houser CR, Jones EG, Vaughn JE (1983) Synaptic organization of immunocytochemically identified GABA neurons in the monkey sensory-motor cortex. J Neurocytol 12:639–660
Hendry SHC, Jones EG, Emson PC, Lawson DEM, Heizmann CW, Streit P (1989) Two classes of cortical GABA neurons defined by differential calcium binding protein immunoreactivities. Exp Brain Res 76:467–472
Houser CR, Hendry SHC, Jones EG, Vaughn JE (1983) Morphological diversity of immunocytochemically identified GABA neurons in the monkey sensory-motor cortex. J Neurocytol 12:617–638
Kägi U, Berchtold MW, Heizmann CW (1987) Ca2+-binding parvalbumin in rat testis. Characterization, localization and expression during development. J Biol Chem 262:7314–7320
Kosaka T, Heizmann CW, Tateishi K, Hamaoka Y, Hama K (1987) An aspect of the organizational principle of the γ-aminobutyric acid ergic system in the cerebral cortex. Brain Res 409:403–408
Lotstra F, Vanderhaeghen JJ (1984) Possible misinterpretations of radioimmunoassay results of endocrine tissues containing peptides in the micromolar range. Med Biol 62:59–63
Mason DY, Abdulaziz Z, Falini B, Stein H (1983) Double immunoenzymatic labelling. In: Polak JM, Van Noorden S (eds) Techniques in immunocytochemistry. PSG Wright, Bristol London Boston, pp 113–128
Naegele JR, Barnstable CJ (1989) Molecular determinants of GABAergic local-circuit neurons in the visual cortex. Trends Neurosci 12:28–34
Nakane PK, Pierce GB (1967) Enzyme-labelled antibodies: preparation and application for the localization of antigens. J Hist Cytochem 14:929–931
Norman AW, Roth J, Orci L (1982) The vitamin D endocrine system: steroid metabolism, hormone receptors, and biological response (calcium binding proteins). Endocr Rev 3:331–336
Orban GA (1984) Neuronal operations in the visual cortex. In: Barlow HB, Bullock TH, Florey E, Grüsser OJ, Peters A (eds) Studies of brain function, Vol 11. Springer, Berlin Heidelberg New York Tokyo
Ribak CE (1978) Aspinous and sparsely spinous stellate neurons in the visual cortex of rats contain glutamic acid decarboxylase. J Neurocytol 7:461–478
Sachs DH, Winn JH (1970) The use of glutaraldehyde as a coupling agent for ribonuclease and bovine serum albumin. Immunochemistry 7:581–585
Sillito AM (1974) Effects of the iontophoretic application of bicuculline on the receptive field properties of simple cells in the visual cortex of the cat. J Physiol (Lond) 242:1279–1289
Sillito AM (1975a) The effectiveness of bicuculline as an antagonist of GABA and visually evoked inhibition in the cat's striate cortex. J Physiol (Lond) 250:287–304
Sillito AM (1975b) The contribution of inhibitory mechanisms to the receptive field properties of neurons in the striate cortex of the cat. J Physiol (Lond) 250:305–329
Sillito AM, Kemp JA, Milson JA, Berardi N (1980) A re-evaluation of the mechanisms underlying simple cell orientation selectivity. Brain Res 194:517–520
Somogyi P, Freund TF, Wu J-Y, Smith AD (1983) The section-Golgi impregnation procedure. 2. Immunocytochemical demonstration of glutamate decarboxylase in Golgi-impregnated neurons and in their afferent synaptic boutons in the visual cortex of the cat. Neuroscience 9:475–490
Somogyi P, Hodgson AJ, Smith AD, Nunzi MG, Gorio A, Wu J-Y (1984) Different populations of GABAergic neurons in the visual cortex and hippocampus of cat contain somatostatinor cholecystokinin-immunoreactive material. J Neurosci 4:2590–2603
Somogyi P, Hodgson AJ (1985) Antiserum to γ-aminobutyric acid. III. Demonstration of GABA in Golgi-impregnated neurons and in convential electron microscopic sections of cat striate cortex. J Hist Cytochem 33:249–257
Somogyi P, Freund TF, Hodgson AJ, Somogyi J, Beroukas D, Chubb IW (1985) Identified axo-axonic cells are immunoreactive for GABA in the hippocampus and visual cortex of the cat. Brain Res 332:143–149
Spencer R, Charman M, Wilson PW, Lawson DEM (1978) The relationship between vitamin D-stimulated calcium transport and intestinal calcium-binding protein in the chicken. Biochem J 170:92–101
Sternberger LA, Hardy PH, Cuculis JJ, Meyer HG (1970) The unlabelled antibody-enzyme method of immunohistochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase anti-horseradish peroxidase) and its use in identification of spirochetes. J Hist Cytochem 18:315–317
Stichel CC, Singer W, Heizmann CW, Norman AW (1987) Immunohistochemical localization of calcium-binding proteins, parvalbumin and calbindin-D 28K, in the adult and developing visual cortex of cats: a light and electron microscopic study. J Comp Neurol 262:563–577
Vandesande F (1983) Immunohistochemical double staining techniques. In: Cuello AC (ed) Immunohistochemistry. Wiley, New York, pp 257–272
Vandesande F, Dierickx K, De Mey J (1977) The origin of the vasopressinergic and oxytocinergic fibres of the median eminence of the rat hypophysis. Cell Tiss Res 180:443–452
Vidyasagar TR, Heide W (1986) The role of GABAergic inhibition in the response properties of neurons in cat visual area 18. Neuroscience 17:49–55
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Demeulemeester, H., Arckens, L., Vandesande, F. et al. Calcium binding proteins and neuropeptides as molecular markers of GABAergic interneurons in the cat visual cortex. Exp Brain Res 84, 538–544 (1991). https://doi.org/10.1007/BF00230966
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DOI: https://doi.org/10.1007/BF00230966