Abstract
The possible effect of several physiologically important aldehydes has been tested on partially purified glyoxalase I of Ehrlich ascites carcinoma (EAC) cells. The results indicate that D, and L-lactaldehyde are strong non-competitive inhibitors of glyoxalase I and the effect with the D-isomer is more pronounced, whereas both D,L-glyceraldehyde and acetaldehyde are moderately inhibitory and the nature of inhibition is strictly competitive. Moreover, D,L-glyceraldehyde strongly inhibits the utilization of methylglyoxal by intact EAC cells. A search for the presence of several aldehyde metabolizing enzymes in EAC cells indicates that non-specific aldehyde reductase, methylglyoxal reductase, aldehyde dehydrogenase and alcohol dehydrogenase are apparently absent in this rapidly growing, highly de-differentiated malignant cell.
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Thornalley PJ: The glyoxalase system: Developments towards functional characterization of a metabolic pathway fundamental to biological life. Biochem J 269: 1–11, 1990
Kalapos MP: Methylglyoxal toxicity in mammals. Toxicol Lett 73: 324, 1994
Racker E: The mechanism of action of glyoxalase. J Biol Chem 190: 685–696, 1951
Együd LG, Szent-Györgyi A: On the regulation of cell division. Proc Nat Acad Sci 56: 203–207, 1966
Szent-Györgyi A, Együd LG, McLaughlin JA: Keto-aldehydes and cell division. Science 155: 539–541, 1967
Együd LG, Szent-Györgyi A: Cancerostatic action of methylglyoxal. Science 160: 1140, 1968
Ranganathan S, Tew KD: Analysis of glyoxalase-I from normal and tumor tissue from human colon. Biochim Biophys Acta 1182: 311–316, 1993
Ray M, Halder J, Dutta SK, Ray S: Inhibition of respiration of tumor cells by methylglyoxal and protection of inhibition by lactaldehyde. Int J Cancer 47: 603–409, 1991
Halder J, Ray M, Ray S: Inhibition of glycolysis and mitochondrial respiration of Ehrlich ascites carcinoma cells by methylglyoxal. Int J Cancer 54: 443–449, 1993
Ray S, Dutta S, Halder J, Ray M: Inhibition of electron flow through complex I of the mitochondrial respiratory chain of Ehrlich ascites carcinoma cells by methylglyoxal. Biochem J 303: 69–72, 1994
Ray M, Ray S: Purification and partial characterization of a methylglyoxal reductase from goat liver. Biochim Biophys Acta 802: 119–127, 1984
Murata K, Fukuda Y, Simosaka M, Watanabe K, Saikusa T, Kimura A: Metabolism of 2-oxoaldehydes in yeasts. Purification and characterization of NADPH-dependent methylglyoxal-reducing enzyme from Saccharomyces cerevisiae. Eur J Biochem 151: 631–436, 1985
Ghoshal K, Banerjee AB, Ray S: Methylglyoxal-catabolizing enzymes of Leishmania donovani promastigotes. Mol Biochem Parasitol 35: 21–30, 1989
Ray S, Ray M: A reinvestigation of inhibitors of glyoxalase l. J Biosci 12: 405–414, 1987
Apple MA, Greenberg DM: Arrest of cancer in mice by therapy with normal metabolites. II. Indefinite survivors among mice treated with mixtures of 2-oxopropanol and 2,3-dihydroxy-propanal. Cancer Chemother Rep 52: 687–696, 1968
Baggetto LG: Deviant energetic metabolism of glycolytic cancer cells. Biochimie 74: 959–974, 1992
Bernardi G: Chromatography of proteins on hydroxyapatite. Methods Enzymol 22: 325–339, 1971
Cooper RA: Methylglyoxal synthase. Methods Enzymol 41: 502–508, 1975
Ray S, Ray M: Oxidation of lactaldehyde by cytosolic aldehyde dehydrogenase and inhibition of cytosolic and mitochondrial aldehyde dehydrogenase by metabolites. Biochim Biophys Acta 802: 128–134, 1984
Mannervik B, Aronsson A-C, Tibbelin G: Glyoxalase 1 from Human Erythrocytes. Methods Enzymol 90: 535–541, 1982
Oray B, Norton SJ: Glyoxalase 11 from mouse liver. Methods Enzymol 90: 547–551, 1982
Racker E: Alcohol dehydrogenase from baker's yeast. Methods Enzymol 1: 500–503, 1955
Layne E: Spectrophotometric and turbidimetric methods for measuring proteins. Methods Enzymol 3: 447–454, 1957
Wermuth B, Munch JDB, von Wartburg JP: Purification and properties of NADPH-dependent aldehyde reductase from human liver. J Biol Chem 252: 3821–3828, 1977
Russo JE, Hilton J: Characterization of cytosolic aldehyde dehydrogenase from cyclophosphamide resistance L1210 cells. Cancer Res 48: 2963–2968, 1988
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Biswas, S., Bhattacharjee, S., Ray, M. et al. Interaction of aldehydes with glyoxalase I and the status of several aldehyde metabolizing enzymes of Ehrlich ascites carcinoma cells. Mol Cell Biochem 165, 9–16 (1996). https://doi.org/10.1007/BF00229740
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DOI: https://doi.org/10.1007/BF00229740