Abstract
Genes encoding high-molecular-weight (HMW) glutenin subunits, present in bread-wheat lines and cultivars, were studied by RFLP (restriction fragment length polymorphism) and PCR (polymerase chain reaction) analyses. In particular, allelic subunits of the x-or y-type, encoded at the Glu-D1 locus present on the long arm of chromosome 1D, were investigated. The variation in size, observed in different allelic subunits, is mainly due to variation in the length of the central repetitive domain, typical of these proteins. Deletions or duplications, probably caused by unequal crossingover, have given rise to the size heterogeneity currently observed. The possibility of using the PCR technique for a detailed analysis of HMW glutenin genes in order to obtain a more accurate estimation of the molecular weight of their encoded subunits, and the detection of unexpressed genes, is also described.
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Anderson OD, Halford NG, Forde J, Yip RE, Shewry PR, Greene FC (1988) Structure and analysis of the high-molecular-weight glutenin genes from Triticum aestivum L. cv Cheyenne. Proc 7th Int Wheat Genet Symp, IPSR, Cambridge, pp 699–704
Anderson OD, Greene FC, Ryan EY, Halford NG, Shewry PR, Malpica-Romero J-M (1989) Nucleotide sequences of the two high-molecular-weight glutenin genes from the D-genome of a hexaploid bread-wheat, Triticum aestivum L. cv Cheyenne. Nucleic Acids Res 17:461–462
Bunce N, White RP, Shewry PR (1985) Variation in estimates of molecular weights of cereal prolamins by SDS-PAGE. J Cereal Sci 3:131–142
D'Ovidio R, Tanzarella OA, Porceddu E (1992) Isolation of an alpha-type gliadin gene from Triticum durum Desf and genetic polymorphism at the Gli-2 loci. J. Genet Breed 46:41–48
D'Ovidio R, Anderson OD, Porceddu E (1993) PCR identification of specific gliadin and glutenin genes in wheat. 5th Int Workshop on Gluten Proteins, Detmold, Germany (in press)
Goldsbrough AP, Bulleid NJ, Freedman RB, Flavell RB (1989) Conformational differences between two wheat (Triticum aestivum) highmolecular-weight glutenin subunits are due to a short region containing six amino-acid differences. Biochem J 263:837–842
Greene FC, Anderson OD, Yip RE, Halford NG, Malpica-Romero J-M, Shewry PR (1988) Analysis of possible quality-related sequence variations in the 1D glutenin high-molecular-weight subunit genes of wheat. Proc 7th Int Wheat Genet Symp, IPSR, Cambridge, pp 735–740
Halford NG, Forde J, Anderson OD, Greene FC, Shewry PR (1987) The nucleotide and deduced amino-acid sequences of a HMW glutenin subunit gene from chromosome 1B of bread-wheat (Triticum aestivum L.) and comparison with those of genes from chromosomes 1A and 1D. Theor Appl Genet 75:117–126
Harberd NP, Bartels D, Thompson RD (1986) DNA restriction fragment variation in the gene family encoding high-molecular-weight (HMW) glutenin subunits of wheat. Biochem Genet 24:579–596
Harberd NP, Flavell RB, Thompson RD (1987) Identification of a transposon-like insertion in a Glu-1 allele of wheat. Mol Gen Genet 209:326–332
Kasarda DD (1989) Glutenin structure in relation to wheat quality. In: Pomeranz Y (ed) Wheat is unique. Am Assoc Cereal Chem, St Paul Minnesota, pp 277–302
Lafiandra D, Benedettelli S, Porceddu E (1988) Null forms for storage proteins in bread-wheat and durum. Proc 7th Int Wheat Genet Symp, IPSR, Cambridge, pp 963–967
Lafiandra D, D'Ovidio R, Porceddu E, Margiotta B, Colaprico G (1993) New data supporting high M r glutenin subunit 5 as a determinant of qualitative differences in the pairs 5+10 vs 2+12. J Cereal Sci 18:197–205
Margiotta B, Colaprico G, D'Ovidio R, Lafiandra D (1993) Characterization of high-M r subunits of glutenin by combined chromatographic and electrophoretic separations and restriction fragment length polymorphism (RFLP) analysis of their encoding genes. J Cereal Sci 17:221–236
Payne PI, Lawrence GJ (1983) Catalogue of alleles for the complex gene loci, GluA1, GluB1, GluD1, which code for the high-molecular-weight subunits of glutenin in hexaploid wheat. Cereal Res Commun 11:29–35
Payne PI (1987) Genetics of wheat storage proteins and the effect of allelic variation on bread-making quality. Annu Rev Plant Physiol 38: 141–153
Payne PI, Law CN, Mudd EE (1980) Control by homoeologous group I chromosomes of the high-molecular-weight subunits of glutenin, a major protein of wheat endosperm. Theor Appl Genet 58:113–120
Payne PI, Corfield KG, Holt LM, Blackman JA (1981) Correlation between the inheritance of certain high-molecular-weight subunits of glutenin and breadmaking quality in progenies of six crosses of bread wheat. J Sci Food Agric 32:51–60
Payne PI, Nightingale MA, Krattiger AF, Holt LM (1987) The relationship between HMW glutenin subunit composition and the breadmaking quality of British-grown wheat varieties. J Sci Food Agric 40:51–65
Shewry PR, Halford NG, Tatham AS (1989) The high-molecularweight subunits of wheat, barley and rye: genetics, molecular biology, chemistry and role in wheat gluten structure and functionality. In: Miflin BJ (ed) Oxford Surveys of Plant Molecular and Cell Biology, vol 6. Oxford University Press, Oxford, pp 163–219
Shewry PR, Halford NG, Tatham AS (1992) High-molecular-weight subunits of wheat glutenin. J Cereal Sci 15:105–120.
Sugiyama T, Rafalski A, Peterson D, Soll D (1985) A wheat HMW glutenin subunit gene reveals a highly repeated structure. Nucleic Acids Res 13:8729–8737
Thompson RD, Bartels D, Harberd NP (1985) Nucleotide sequence of a gene from chromosome 1D of wheat encoding a HMW-glutenin subunit. Nucleic Acids Res 19:6833–6846
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Communicated by H. F. Linskens
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D'Ovidio, R., Porceddu, E. & Lafiandra, D. PCR analysis of genes encoding allelic variants of high-molecular-weight glutenin subunits at the Glu-D1 locus. Theoret. Appl. Genetics 88, 175–180 (1994). https://doi.org/10.1007/BF00225894
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DOI: https://doi.org/10.1007/BF00225894