Abstract
A population of 169 microspore-derived doubled-haploid lines was produced from a highly polymorphic Brassica oleracea cross. A dense genetic linkage map of B. oleracea was then developed based on the segregation of 303 RFLP-defined loci. It is hoped that these lines will be used by other geneticists to facilitate the construction of a unified genetic map of B. oleracea. When the B. oleracea map was compared to one ofB. napus (Parkin et al. 1995), based on the same RFLP probes (Sharpe et al. 1995), good collinearity between the C-genome linkage groups of the two species was observed.
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References
Camargo LEA, Osborn TC (1996) Mapping loci controlling flowering time in Brassica oleracea. Theor Appl Genet 92:610–616
Camargo LEA, Savides L, Jung G, Nienhuis J, Osborn TC (1996) Location of the self-incompatibility locus in an RFLP and RAPD map of Brassica oleracea. Heredity (in press)
Chuong PV, Beversdorf WD (1985) High-frequency embryogenesis through isolated microspore culture in Brassica napus L. and Brassica carinata Braun. Plant Sci 39:219–226
Ferreira ME, Williams PH, Osborn TC (1994) RFLP mapping of Brassica napus using doubled-haploid lines. Theor Appl Genet 89:615–621
Hu J, Quiros CF (1991) Molecular and cytological evidence of deletions in alien chromosomes for two monosomic addition lines of Brassica campestris-oleracea. Theor Appl Genet 81:221–226
Kianian SF, Quiros CF (1992) Generation of a Brassica oleracea composite RFLP map: linkage arrangements among various populations and evolutionary implications. Theor Appl Genet 84:544–554
Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175
Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) Mapmaker; an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181
Landry BS, Hubert N, Crete R, Chang MS, Lincoln SE, Etoh T (1991) A genetic map of Brassica napus based on restriction fragment length polymorphism detected with expressed DNA sequences. Genome 34:543–552
Landry BS, Hubert N, Crete R, Chang MS, Lincoln SE, Etoh T (1992) A genetic map for Brassica oleracea based on RFLP markers detected with expressed DNA sequences and mapping of resistance genes to race 2 of Plasmodiophora brassicae (Woronin). Genome 35:409–420
Lichter R, De Groot E, Fiebig D, Schweiger R, Gland A (1988) Glucosinolates determined by HPLC in seeds of microspore-derived homozygous lines of rapeseed (Brassica napus L.). Plant Breed 100:209–221
McGrath JM, Quiros CF, Harada JJ, Landry BS (1990) Identification of Brassica oleracea monosomic alien chromosome addition lines with molecular markers reveals extensive gene duplication. Mol Gen Genet 223:198–204
Orton TJ, Browers MA (1985) Segregation of genetic markers among plants regenerated from cultured anthers of broccoli (Brassica oleracea var. italica). Theor Appl Genet 69:637–643
Parkin AP, Sharpe AG, Keith DJ, Lydiate DJ (1995) Identification of the A and C genomes of the amphidiploid Brassica napus (oilseed rape). Genome 38:1122–1131
Sharpe AG, Parkin AP, Keith DJ, Lydiate DJ (1995) Frequent non reciprocal translocations in the amphidiploid genome of oilseed rape (Brassica napus). Genome 38:1112–1121
Slocum MK, Figdore SS, Kennard WC, Suzuki JY, Osborn TC (1990) Linkage arrangement of restriction fragment length polymorphism loci in Brassica oleracea. Theor Appl Genet 80:57–64
Teutonico RA, Osborn TC (1994) Mapping of RFLP and qualitative trait loci in Brassica rapa and comparison to the linkage maps of B. napus, B. oleracea, and Arabidopsis thaliana. Theor Appl Genet 89:885–894
Thormann CE, Ferreira ME, Camargo LEA, Tivang JG, Osborn TC (1994) Comparison of RFLP and RAPD markers to estimating genetic relationships within and among cruciferous species. Theor Appl Genet 88:973–980
U N (1935) Genome analysis in Brassica with special reference to the experimental formation of B. napus and peculiar mode of fertilization. Jpn J Bot 7:389–452
Uzunova M, Ecke W, Weissleder K, Robbelen G (1995) Mapping the genome of rapeseed (Brassica napus L.). I. Construction of an RFLP linkage map and localization of QTLs for seed glucosinolate content. Theor Appl Genet 90:194–204
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Communicated by G. Wenzel
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Bohuon, E.J.R., Keith, D.J., Parkin, I.A.P. et al. Alignment of the conserved C genomes of Brassica oleracea and Brassica napus . Theoret. Appl. Genetics 93, 833–839 (1996). https://doi.org/10.1007/BF00224083
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DOI: https://doi.org/10.1007/BF00224083