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Kinetic studies of acetate fermentation by Methanosarcina sp. MSTA-1

  • Environmental Microbiology
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Summary

The effect of three parameters (initial acetate concentration, temperature and pH) on the acetoclastic reaction was studied with the thermophilic methanogenic bacterium Methanosarcina sp. MSTA-1. The optimum temperature for growth ranged around 55° C, and optimum pH was 6.5–7.5, giving a minimum generation time of 12.6–13.9 h (µmax = 0.050–0.055 h−1) and a maximum value of the specific acetate consumption rate (q suppsinfs ) of 14–20 mmol/g cells per hour. Contrary to the methane yield, the growth yield was found to be dependent on culture conditions, especially on incubation temperature. Methanosarcina sp. MSTA-1 showed a low affinity for acetate substrate. Growth at 55° C and at constant pH 7 resulted in a K m value and a threshold acetate concentration of 10.7 mM and 0.7 mM, respectively.

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  1. Laboratoire de Biotechnologie de l'Environnement des Industries Agroalimentaires, Institut National de la Recherche Agronomique, Boulevard du General de Gaulle, BP 429, F-11 104, Narbonne Cedex, France

    M. Clarens & R. Molleta

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  1. M. Clarens
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  2. R. Molleta
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Clarens, M., Molleta, R. Kinetic studies of acetate fermentation by Methanosarcina sp. MSTA-1. Appl Microbiol Biotechnol 33, 239–244 (1990). https://doi.org/10.1007/BF00176532

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  • DOI: https://doi.org/10.1007/BF00176532

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