Abstract
The levels of total protein in tears from healthy donors, conjunctivitis vernalis patients, and conjunctivitis follicularis patients, were 625, 1370 and 1160 mg% respectively. Serum albumin accounted for 3.3%, 43% and 67% of the total protein of tears from these groups, and the level of proteins probably synthesized by the lacrimal gland, was in tears from conjunctivitis follicularis patients only half the level in normal tears. By crossed immunoelectrophoresis with intermediate gel, 10 antigenic species could be recognized in normal tears, and of these the following were identified: Lysozyme, IgA, lactoferrin and serum abumin. In tears from patients with conjuntivitis vernalis three more immunoprecipitates were observed, of which one was due to IgG. No lysozyme could be demonstrated in tears from a case of conjunctivitis sicca by immunoelectrophoresis.
In tears from healthy donors the mean level of IgA was 20 mg%, of IgG 3 mg%, and IgM could not be demonstrated. Rabbit anti-tear immunoglobulin did not precipitate a standard of human IgM in double immunodiffusion. In cases of conjunctivitis vernalis and follicularis the mean levels were increased to 80 and 114 mg% IgG, and 11 and 14 mg% IgM, but IgA was increased only to 32 and 41 mg%. It is assumed that the level of IgA in normal tears is almost entirely due to local synthesis, while serum albumin and other immunoglobulins may have escaped from the circulation by molecular sieving. The increased levels of immunoglobulins in inflammatory diseases is probably due to transudation. However, in blepharoconjunctivitis patients several tear samples with a high IgM and a low or zero level of IgG could be demonstrated. Possible explanations for this phenomenon are discussed.
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References
Allansmith, M. Immunology of the tears. Ophthal. Clin. 13: 47–72 (1973).
Axelsen, N.H. Intermediate gel in crossed and fused rocket immunoelectrophoresis. Scand. J. Immunol. 2, Suppl. 1: 71–77 (1973).
Bluestone, R., Easty, D.L., Goldberg, L.S., Jones, B.R. & Pettit, T.H. Lacrimal immunoglobulins and complement quantified by counterimmunoelectrophoresis. Brit. J. Ophthal. 59: 279–281 (1975).
Bock, E. & Axelsen, N.H. Comparison of antigens: the reaction of partial identity. Scand. J. Immunol. 2, Suppl. 1: 95–99 (1973).
Bonavida, B., Sapse, A.T. & Sercarz, E.E. Specific tear prealbumin. A unique lachrymal protein absent from serum and other secretions. Nature (London) 221: 375–378 (1969).
Eylan, E., Ronen, D., Romano, A. & Smetana, O. Lysozyme tear levels in patients with herpes simplex virus eye infection. Invest. Ophthal. 16: 850–853 (1977).
Gachon, A.M., Verrelle, P., Betail, G. & Dastugue, B. Immunological and electrophoretic studies of human tear proteins. Exp. Eye Res. 29: 539–553 (1979).
Hartree, E.F. Determination of protein. A modification of the Lowry method that gives a linear photometric response. Anal. Biochem. 48: 422–427 (1972).
Josephson, A.S. & Lockwood, D.W. Immunoelectrophoretic studies of the protein components of normal tears. J. Immunol. 93: 532–539 (1964).
Josephson, A.S. & Weiner, R.S. Studies of the proteins of lacrimal secretions. Immunology 100: 1080–1092 (1968).
Little, J.M., Centifanto, Y.M. & Kaufman, H.E. Immunoglobulins in tears. Amer. J. Ophthal. 68: 898–905 (1969).
Mancini, G., Barbonara, A.O. & Heremans J.F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry 2: 235–254 (1965).
McClellan, B.H., Whitney, C.R., Newman, L.P. & Allansmith, M.R. Immunoglobulin in tears. Amer. J. Ophthal. 76: 89–101 (1973).
Moses, R.A. Adler's Physiology of the Eye. Clinical Application, 6th ed. St. Louis, Mosby, 1975, p. 20.
Ronen, D., Eylan, E., Romano, A., Stein, R. & Modan, M. A spectrophotometric method for quantitative determination of lysozyme in human tears: description and evaluation of the method and screening of 60 healthy subjects. Invest. Ophthal. 14: 479–484 (1975).
Sapse, A.T., Bonavida, B., Stone, W., Jr., Barnett, E.V. & Sercarz, E.E. La détermination quantitative des protéines dans les larmes humaines. Bull. Soc. Fr. Ophtal. 80: 236–248 (1967).
Sen, D.K. & Sarin, G.S. Immunoglobulin concentrations in human tears in ocular diseases. Brit. J. Ophthal. 63: 297–800 (1979).
Smolin, G. & Okumoto, M. Staphylococcal blepharitis. Arch. Ophthal. 95: 812–816 (1977).
Tomasi, T.B., Jr. The Immune System of Secretions. Englewood Cliffs, N.J., 1976, p. 65.
West, E.S., Todd, W.R., Mason, H.S. & Van Bruggen, J.T. Textbook of Biochemistry, 4th ed. New York, Macmillan, 1961, p. 625.
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Zavaro, A., Samra, Z., Baryishak, R. et al. Proteins in tears from healthy and diseased eyes. Doc Ophthalmol 50, 185–199 (1980). https://doi.org/10.1007/BF00161161
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DOI: https://doi.org/10.1007/BF00161161