Abstract
The disruption of commercially-available pressed Bakers' yeast (Saccharomyces cerevisiae) was studied using a relatively new high-pressure homogenizer (the Microfluidizer). Initial experiments using only mechanical disruption generally gave low disruption yields (i.e., less than 40% disruption in 5 passes). Consequently combinations of two disruption methods, namely enzymatic lysis and subsequent homogenization, were tested to identify achievable levels of disruption. The enzyme preparation employed was Zymolyase, which has been shown to effectively lyse the walls of viable yeast. Yeast cell suspensions ranging in concentration from 0.6 to 15 gDW/L were disrupted with and without enzymatic pre-treatment. Final total disruption obtained using the combined protocol approached 100% with 4 passes at a pressure of 95 MPa, as compared to only 32% disruption with 4 passes at 95 MPa using only homogenization. A model is presented to predict the fraction disrupted while employing this novel enzymatic pretreatment.
Similar content being viewed by others
Abbreviations
- a:
-
exponent of pressure (-)
- b:
-
exponent of number of passes (-)
- K:
-
disruption constant (MPa-a)
- N:
-
number of passes (-)
- P:
-
pressure (MPa)
- R:
-
total fraction of cells disrupted (-)
- Ro:
-
fraction of cells disrupted after enzymatic pre-treatment (-)
- X:
-
cell concentration (dry weight) (gDW/L)
- DW:
-
dry weight
References
M. White and D. Marcus, “Disintegration of Microorganisms”, in Downstream Processes: Equipment and Techniques, A. Mizahi, Ed., Alan R. Liss, Inc., New York, (1988), p. 52.
T. Sauer, C.W. Robinson, B. Glick, Biotechnol. Bioeng., 33, 1330 (1989).
K. Kitamura and Y. Yamamoto, Arch. Biochem. Biophys., 153, 403 (1972).
T. Kaneko, K. Kitamura, Y. Yamamoto, Agric. Biol. Chem., 37, 2295 (1973).
K. Kitamura, Agric. Biol. Chem., 46, 963 (1982).
K. Kitamura, Agric. Biol. Chem., 46, 2093 (1982).
K. Kitamura, T. Kaneko, Y. Yamamoto, Arch. Biochem. Biophys., 145, 402 (1971).
K. Kitamura, T. Kaneko, Y. Yamamoto, J. Gen. Appl. Microbiol., 18, 57 (1972).
Product Information Bulletin #120491, Seikagaku Kogyo Co., Ltd., Tokyo, Japan.
P. Hetherington, M. Follows, P. Dunnill, M. Lilly, Trans. Inst. Chem. Eng., 49, 142 (1971).
J. Brookman, Biotechnol. Bioeng., 16, 371 (1974).
C. Engler and C.W. Robinson, Biotechnol. Bioeng., 23, 765 (1981).
P. Dunnill and M. Lilly, Biotechnol. Bioeng. Symp., 3, 97 (1972).
D. Whitworth, Bioetechnol. Bioeng., 16, 1399 (1974).
M. Doulah, T. Hammond, J. Brookman, Biotechnol. Bioeng., 17, 845 (1975).
C. Engler and C.W. Robinson, Biotechnol. Bioeng., 21, 1861 (1979).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Baldwin, C., Robinson, C.W. Disruption of Saccharomyces cerevisiae using enzymatic lysis combined with high-pressure homogenization. Biotechnol Tech 4, 329–334 (1990). https://doi.org/10.1007/BF00157431
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00157431