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HPLC analysis of tear proteins in giant papillary conjunctivitis

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Abstract

Giant papillary conjunctivitis (GPC) is a well defined entity seen in patients with deposits on their lenses. High performance liquid chromatography (HPLC) tear protein analysis was performed on 17 GPC patients and compared with healthy controls with and without contact lenses. The IgA levels are somewhat lower in GPC patients as compared with healthy controls not wearing lenses (P < 0.05). A similar decrease was also seen in the control group wearing contact lenses. We were not able to identify a specific HPLC tear protein profile in GPC patients as compared with controls. The HPLC profile therefore gives no explanation for the lens deposits and is not suitable as a diagnostic or prognostic tool.

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References

  1. Allansmith MR, Korb DR, Greiner JV, Henriquez AS, Simon MA, Rinnemore VM. Giant papillary conjunctivitis in contact lens wearers. Am J Ophthalmol 1977; 83: 697–708.

    Google Scholar 

  2. Ballow M, Donshik PC, Mendelson L. Complement proteins and C3 anaphylatoxin in the tears of patients with conjunctivitis. J Allergy Clin Immunol 1985; 76: 473–476.

    Google Scholar 

  3. Ballow M, Donshik PC, Rapacz P, Samartino L. Tear lactoferrin levels in patients with external inflammatory ocular disease. Invest Ophthalmol Vis Sci 1987; 28: 543–545.

    Google Scholar 

  4. Boonstra A, Kijlstra A. Separation of human tear proteins by high performance liquid chromatography. Curr Eye Res 1984; 3: 1461–1469.

    Google Scholar 

  5. Boonstra A, van Haeringen N, Kijlstra A. Human tears inhibit the coating of proteins to solid surfaces. Curr Eye Res 1985; 4: 1137–1143.

    Google Scholar 

  6. Boukes RJ, Boonstra A, Breebaart AC, Reits D, Glasius E, Luyendijk L, Kijlstra A. Analysis of human tear protein profiles using high performance liquid chromatography. Doc Ophthalmol 1987; 67: 105–113.

    Google Scholar 

  7. Donshik PC, Ballow M. Tear immunoglobulins in giant papillary conjunctivitis induced by contact lenses. Am J Ophthalmol 1983; 96: 460–466.

    Google Scholar 

  8. Fowler SA, Allansmith MR. Evolution of soft contact lens coatings. Arch Ophthalmol 1980; 98: 95–99.

    Google Scholar 

  9. Gudmundsson OG, Woodward DF, Fowler SA, Allansmith MR. Identification of proteins in contact lens surface deposits by immunofluorescence microscopy. Arch Ophthalmol 1985; 103: 196–197.

    Google Scholar 

  10. van Haeringen NJ. Clinical biochemistry of tears. Surv Ophthalmol 1981; 26: 84–96.

    Google Scholar 

  11. Polak BCP, Kijlstra A, Luyendijk L, Boonstra A. Analysis of tears in contact lens wearers using HPLC. Ophthalmology Today, Elsevier Science Publishers 1988; 635–636.

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Suttorp-Schulten, M.S.A., Luyendijk, L., Kok, J.H.C. et al. HPLC analysis of tear proteins in giant papillary conjunctivitis. Doc Ophthalmol 72, 235–240 (1989). https://doi.org/10.1007/BF00153490

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  • DOI: https://doi.org/10.1007/BF00153490

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