Abstract
An efficient tissue culture system for high frequency of plant regeneration from hypocotyl explants of Brassica carinata was developed via manipulation of culture medium and selection of explants. Explants grown on medium containing combinations of 2 mg l-1 BA and 0.01 mg l-1 NAA or 4 mg l-1 kinetin and 0.01 mg l-1 2,4-D regenerated shoots at 100% frequency. High frequency shoot regeneration occurred only from explants originating from 6 to 7-day-old but not younger or older seedlings. Explants showed higher regeneration capacity at the distal end than the proximal end, and the upper segment was more regenerative than the lower segment of hypocotyl. Regenerants were rooted on half-strength growth regulator-free medium, acclimatized and developed into normal, fertile plants.
Similar content being viewed by others
Abbreviations
- BA:
-
benzyladenine
- 2-4-D:
-
2,4-dichlorophenoxyacetic acid
- NAA:
-
naphthaleneacetic acid
- MS:
-
Murashige & Skoog
References
Anand IJ, Singh JP & Malik RS (1985) B. carinata — A potential oil seed crop for rainfed agriculture. Cruciferae Newsl. 10: 76–79
Chi G-L & Pua E-C (1989) Ethylene inhibitors enhanced de novo shoot regeneration from cotyledons of Brassica campestris ssp. chinensis in vitro. Plant Sci. 64: 243–250
Chuong PV, Pauls KP & Beversdorf WD (1987) Protoplast culture and plant regeneration from Brassica carinata Braun. Plant Cell Rep. 6: 67–69
Dietert MF, Barron SA & Yoder OC (1982) Effects of genotype on in vitro culture in the genus Brassica. Plant Sci. Lett. 26: 233–240
Jourdan PS & Earle ED (1989) Genotypic variability in the frequency of plant regeneration from leaf protoplasts of four Brassica spp. and Raphanus sativus. J. Am. Soc. Hortic. Sci. 114: 343–349
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Narasimhulu SB & Chopra VL (1987) Plant regeneration from callus cultures of Brassica carinata A. Br. and its implications to improvement of oil seed Brassicas. Plant Breeding 99: 49–55
Narasimhulu SB & Chopra VL (1988) Species specific shoot regeneration response of cotyledonary explants of Brassicas. Plant Cell Rep. 7: 104–106
Narasimhulu SB, Prakash S & Chopra VL (1988) Comparative shoot regeneration responses of diploid Brassicas and their synthetic amphidiploid products. Plant Cell Rep. 7: 525–527
Pua E-C, Mehra-Palta A, Nagy F & Chua N-H (1987) Transgenic plants of Brassica napus L. Bio/Technology 5: 815–817
Pua E-C, Trinh TH & Chua N-H (1989) High frequency plant regeneration from stem explants of Brassica alboglabra Bailey in vitro. Plant Cell Tiss. Org. Cult. 17: 143–152
Yang M-Z & Jia S-R (1989) Plant regeneration from protoplasts of Brassica carinata Braun. Acta Bot. Sin 31: 89–94
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yang, MZ., Jia, SR. & Pua, EC. High frequency of plant regeneration from hypocotyl explants of Brassica carinata A. Br.. Plant Cell Tiss Organ Cult 24, 79–82 (1991). https://doi.org/10.1007/BF00039734
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00039734