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Cloning and characterization of a cDNA encoding a mRNA rapidly-induced by ABA in barley aleurone layers

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Abstract

Abscisic acid (ABA) inhibits the gibberellic acid induced synthesis of α-amylase in barley aleurone layers, yet ABA itself induces more than a dozen polypeptides (Lin & Ho, Plant Physiol 82: 289–297, 1986). As part of our effort to elucidate the molecular action of ABA in barley aleurone layers, we have isolated and characterized an ABA-induced cDNA clone, pHV A1. This cDNA clone hybridizes to an RNA species of approximately 1.1 kb from ABA-treated barley aleurone layers. The level of this mRNA is tripled within 40 minutes after ABA treatment, reaches a peak at 8–12 h, and is present up to 48 h. The induction of this mRNA responds to concentrations of ABA as low as 10-9 M, but higher ABA concentrations induce higher expression of this mRNA. The products of hybrid-select translation and in vitro transcription/translation with pHV A1 comigrate on SDS gel as a 27 kDa polypeptide. However, the sequence of pHV A1 indicates that it has an open reading frame encoding a 22 kDa protein. This size discrepancy is probably due to the high content of the basic amino acid, lysine. This notion has been confirmed by two-dimensional gel electrophoresis showing that this polypeptide is one of the most basic proteins in ABA-treated barley aleurone layers. The deduced amino acid sequence of pHV A1 contains nine imperfect repeats 11 amino acids long which share homology with cotton Lea 7 protein (Baker, Steele & Dure, Plant Mol Biol, in press). The identity and function of the encoded product of pHV A1 is under investigation.

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Authors and Affiliations

  1. Plant Biology Program, Department of Biology, Division of Biology and Biomedical Sciences, Washington University, 63130, St. Louis, MO, USA

    Bimei Hong, Scott J. Uknes & Tuan-hua David Ho

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  1. Bimei Hong
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  2. Scott J. Uknes
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  3. Tuan-hua David Ho
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Hong, B., Uknes, S.J. & Ho, Th.D. Cloning and characterization of a cDNA encoding a mRNA rapidly-induced by ABA in barley aleurone layers. Plant Mol Biol 11, 495–506 (1988). https://doi.org/10.1007/BF00039030

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  • DOI: https://doi.org/10.1007/BF00039030

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