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Agar and ammonium nitrate influence hyperhydricity, tissue nitrate and total nitrogen content of serviceberry (Amelanchier arborea) shoots in vitro

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Abstract

Shoot tip cultures of Amelanchier arborea Michx.f. were grown on Murashige & Skoog or Woody Plant (WP) medium containing 4.4 μM benzyladenine and various concentrations of agar. Increases in agar concentration affected various culture growth variables, decreased culture hyperhydricity and increased tissue nitrate concentration. Additions of ammonium nitrate to cultures grown on WP medium containing 0.4% agar increased all growth variables measured except percent dry weight. Hyperhydricity and tissue nitrate concentration also increase in response to increasing ammonium nitrate in the medium. Since hyperhydricity was shown to be both positively and negatively correlated with increases in tissue nitrate content, it is unlikely that tissue nitrate level alone directly affects hyperhydricity.

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Abbreviations

BA:

benzyladenine

MS:

Murashige & Skoog

WP:

Woody Plant

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Authors and Affiliations

  1. Department of Plant Science, U-67, The University of Connecticut, 06269-4067, Storrs, CT, USA

    Mark H. Brand

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  1. Mark H. Brand
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Brand, M.H. Agar and ammonium nitrate influence hyperhydricity, tissue nitrate and total nitrogen content of serviceberry (Amelanchier arborea) shoots in vitro . Plant Cell Tiss Organ Cult 35, 203–209 (1993). https://doi.org/10.1007/BF00037271

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  • DOI: https://doi.org/10.1007/BF00037271

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