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Simultaneous measurement of changes in red and blue fluorescence in illuminated isolated chloroplasts and leaf pieces: The contribution of NADPH to the blue fluorescence signal

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Abstract

A newly developed nitrogen laser fluorimeter insensitive to actinic illumination was used to follow simultaneously the light induced changes in red and blue fluorescence of intact isolated spinach chloroplasts and leaf pieces. The recorded variable blue fluorescence was linked to a water soluble component of intact isolated chloroplasts, depended on Photosystem I, and was related to changes in carbon metabolism. From the comparison of changes in intact and broken chloroplasts and from fluorescence spectra under different conditions, it was concluded that the variation in NADPH was the major cause for the changes in blue fluorescence. This study opens a path towards continuous and non-destructive monitoring of NADPH redox state in chloroplasts and leaves.

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Abbreviations

Chl:

chlorophyll

DHAP:

dihydroxyacetone phosphate

DLGA:

DL-glyceraldehyde

FNR:

ferredoxin-NADP reductase

FWHM:

full width at half maximum

LED:

light emitting diodes

OAA:

oxaloacetate

qN :

non-photochemical quenching

PGA:

3-phosphoglycerate

Pi:

inorganic orthophosphate

qP :

photochemical quenching

PPFD:

photosynthetic photon flux density

QA :

primary quinone acceptor of Photosystem II

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Authors and Affiliations

  1. Laboratoire pour l'Utilisation du Rayonnement Electromagnetique, Université de Paris XI, 91405, Orsay, France

    Zoran G. Cerovic, Maurice Bergher, Yves Goulas, Stephane Tosti & Ismael Moya

Authors
  1. Zoran G. Cerovic
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  2. Maurice Bergher
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  3. Yves Goulas
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  4. Stephane Tosti
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  5. Ismael Moya
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Cerovic, Z.G., Bergher, M., Goulas, Y. et al. Simultaneous measurement of changes in red and blue fluorescence in illuminated isolated chloroplasts and leaf pieces: The contribution of NADPH to the blue fluorescence signal. Photosynth Res 36, 193–204 (1993). https://doi.org/10.1007/BF00033038

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  • DOI: https://doi.org/10.1007/BF00033038

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